Determination of the DNA content of human chromosomes by flow cytometry

Peter C Harris, E. Boyd, B. D. Young, M. A. Ferguson-Smith

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The mean relative DNA content of each human chromosome was calculated from flow karyotypes of ethidium bromide-stained chromosomes obtained from healthy, normal individuals. These values were found to correlate closely with previously published data obtained by photometric scanning of stained, fixed chromosomes. Calculations of the normal variation in DNA content of each human chromosome indicated that chromosomes 1, 9, 16, and Y (chromosomes with large centric heterochromatic regions) were the most variable, followed by the acrocentrics, 13, 14, 15, 21, and 22. Chromosomes 2, 3, 18, and 19 were also found to vary significantly in DNA content. Chromosomes from a number of subjects with extreme heteromorphisms were flow karyotyped to obtain an estimate of the extent of variation in DNA content of each chromosome. The greatest difference between extreme variants was found for chromosome 1 (which differed by 0.82% of the total genomic DNA), followed by 16 and 9. The largest Y-chromosome variant was 85.9% bigger than the smallest. The precise karyotype analysis produced by flow cytometry resolved many differences between chromosome homologs, including some that cannot be readily distinguished cytogenetically. The implications of these findings for detection of chromosome abnormalities by flow karyotype analaysis are discussed.

Original languageEnglish (US)
Pages (from-to)14-21
Number of pages8
JournalCytogenetics and Cell Genetics
Volume41
Issue number1
StatePublished - 1986
Externally publishedYes

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Human Chromosomes
Flow Cytometry
Chromosomes
Karyotype
DNA
Chromosomes, Human, Pair 1
Y Chromosome
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 2
Ethidium
Chromosome Aberrations

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Harris, P. C., Boyd, E., Young, B. D., & Ferguson-Smith, M. A. (1986). Determination of the DNA content of human chromosomes by flow cytometry. Cytogenetics and Cell Genetics, 41(1), 14-21.

Determination of the DNA content of human chromosomes by flow cytometry. / Harris, Peter C; Boyd, E.; Young, B. D.; Ferguson-Smith, M. A.

In: Cytogenetics and Cell Genetics, Vol. 41, No. 1, 1986, p. 14-21.

Research output: Contribution to journalArticle

Harris, PC, Boyd, E, Young, BD & Ferguson-Smith, MA 1986, 'Determination of the DNA content of human chromosomes by flow cytometry', Cytogenetics and Cell Genetics, vol. 41, no. 1, pp. 14-21.
Harris, Peter C ; Boyd, E. ; Young, B. D. ; Ferguson-Smith, M. A. / Determination of the DNA content of human chromosomes by flow cytometry. In: Cytogenetics and Cell Genetics. 1986 ; Vol. 41, No. 1. pp. 14-21.
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