Determination of 14C radioactivity in ketone bodies

a new, simplified method and its validation

J. M. Miles, M. W. Haymond, R. A. Rizza, J. E. Gerich

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A rapid and reproducible method for the assay of 14C radioactivity in ketone bodies is described, based on the heat lability of acetoacetate and the ability of β-hydroxy-butyrate to quantitatively bind to and elute from an ion exchange resin. Potential interference from 14C activity in the ketone body metabolites (CO2, bicarbonate and acetone) during infusion of 14C-labeled ketone bodies is eliminated. The method was validated both by in vitro recovery studies and by the determination of rates of appearance of ketone bodies during infusion of exogenous unlabeled β-hydroxybutyrate in dogs. The technique avoids, many of the potential sources of imprecision in previous methods and makes in vivo study of ketone body turnover more practical.

Original languageEnglish (US)
Pages (from-to)646-650
Number of pages5
JournalJournal of Lipid Research
Volume21
Issue number5
StatePublished - 1980

Fingerprint

Ketone Bodies
Radioactivity
Hydroxybutyrates
Ion Exchange Resins
Butyrates
Bicarbonates
Metabolites
Acetone
Assays
Hot Temperature
Dogs
Recovery

ASJC Scopus subject areas

  • Endocrinology

Cite this

Miles, J. M., Haymond, M. W., Rizza, R. A., & Gerich, J. E. (1980). Determination of 14C radioactivity in ketone bodies: a new, simplified method and its validation. Journal of Lipid Research, 21(5), 646-650.

Determination of 14C radioactivity in ketone bodies : a new, simplified method and its validation. / Miles, J. M.; Haymond, M. W.; Rizza, R. A.; Gerich, J. E.

In: Journal of Lipid Research, Vol. 21, No. 5, 1980, p. 646-650.

Research output: Contribution to journalArticle

Miles, JM, Haymond, MW, Rizza, RA & Gerich, JE 1980, 'Determination of 14C radioactivity in ketone bodies: a new, simplified method and its validation', Journal of Lipid Research, vol. 21, no. 5, pp. 646-650.
Miles, J. M. ; Haymond, M. W. ; Rizza, R. A. ; Gerich, J. E. / Determination of 14C radioactivity in ketone bodies : a new, simplified method and its validation. In: Journal of Lipid Research. 1980 ; Vol. 21, No. 5. pp. 646-650.
@article{f273a72ddad34420992f063f9755f9d6,
title = "Determination of 14C radioactivity in ketone bodies: a new, simplified method and its validation",
abstract = "A rapid and reproducible method for the assay of 14C radioactivity in ketone bodies is described, based on the heat lability of acetoacetate and the ability of β-hydroxy-butyrate to quantitatively bind to and elute from an ion exchange resin. Potential interference from 14C activity in the ketone body metabolites (CO2, bicarbonate and acetone) during infusion of 14C-labeled ketone bodies is eliminated. The method was validated both by in vitro recovery studies and by the determination of rates of appearance of ketone bodies during infusion of exogenous unlabeled β-hydroxybutyrate in dogs. The technique avoids, many of the potential sources of imprecision in previous methods and makes in vivo study of ketone body turnover more practical.",
author = "Miles, {J. M.} and Haymond, {M. W.} and Rizza, {R. A.} and Gerich, {J. E.}",
year = "1980",
language = "English (US)",
volume = "21",
pages = "646--650",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

}

TY - JOUR

T1 - Determination of 14C radioactivity in ketone bodies

T2 - a new, simplified method and its validation

AU - Miles, J. M.

AU - Haymond, M. W.

AU - Rizza, R. A.

AU - Gerich, J. E.

PY - 1980

Y1 - 1980

N2 - A rapid and reproducible method for the assay of 14C radioactivity in ketone bodies is described, based on the heat lability of acetoacetate and the ability of β-hydroxy-butyrate to quantitatively bind to and elute from an ion exchange resin. Potential interference from 14C activity in the ketone body metabolites (CO2, bicarbonate and acetone) during infusion of 14C-labeled ketone bodies is eliminated. The method was validated both by in vitro recovery studies and by the determination of rates of appearance of ketone bodies during infusion of exogenous unlabeled β-hydroxybutyrate in dogs. The technique avoids, many of the potential sources of imprecision in previous methods and makes in vivo study of ketone body turnover more practical.

AB - A rapid and reproducible method for the assay of 14C radioactivity in ketone bodies is described, based on the heat lability of acetoacetate and the ability of β-hydroxy-butyrate to quantitatively bind to and elute from an ion exchange resin. Potential interference from 14C activity in the ketone body metabolites (CO2, bicarbonate and acetone) during infusion of 14C-labeled ketone bodies is eliminated. The method was validated both by in vitro recovery studies and by the determination of rates of appearance of ketone bodies during infusion of exogenous unlabeled β-hydroxybutyrate in dogs. The technique avoids, many of the potential sources of imprecision in previous methods and makes in vivo study of ketone body turnover more practical.

UR - http://www.scopus.com/inward/record.url?scp=0019226598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019226598&partnerID=8YFLogxK

M3 - Article

VL - 21

SP - 646

EP - 650

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 5

ER -