Determination of neurotransmitters and their metabolites using one- and two-dimensional liquid chromatography with acidic potassium permanganate chemiluminescence detection

Brendan J. Holland, Xavier A. Conlan, Paul G. Stevenson, Susannah J Tye, Ashlie Reker, Neil W. Barnett, Jacqui L. Adcock, Paul S. Francis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

High-performance liquid chromatography with chemiluminescence detection based on the reaction with acidic potassium permanganate and formaldehyde was explored for the determination of neurotransmitters and their metabolites. The neurotransmitters norepinephrine and dopamine were quantified in the left and right hemispheres of rat hippocampus, nucleus accumbens and prefrontal cortex, and the metabolites vanillylmandelic acid, 3,4-dihydrophenylacetic acid, 5-hydroxyindole-3-acetic acid and homovanillic acid were identified in human urine. Under optimised chemiluminescence reagent conditions, the limits of detection for these analytes ranged from 2.5 × 10(-8) to 2.5 × 10(-7) M. For the determination of neurotransmitter metabolites in urine, a two-dimensional high-performance liquid chromatography (2D-HPLC) separation operated in heart-cutting mode was developed to overcome the peak capacity limitations of the one-dimensional separation. This approach provided the greater separation power of 2D-HPLC with analysis times comparable to conventional one-dimensional separations.

Original languageEnglish (US)
Pages (from-to)5669-5676
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume406
Issue number23
DOIs
StatePublished - Sep 1 2014
Externally publishedYes

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Potassium Permanganate
Chemiluminescence
Liquid chromatography
Metabolites
Luminescence
Liquid Chromatography
Neurotransmitter Agents
High Pressure Liquid Chromatography
High performance liquid chromatography
Vanilmandelic Acid
Urine
Homovanillic Acid
Nucleus Accumbens
Prefrontal Cortex
Acetic Acid
Formaldehyde
Limit of Detection
Rats
Hippocampus
Dopamine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Determination of neurotransmitters and their metabolites using one- and two-dimensional liquid chromatography with acidic potassium permanganate chemiluminescence detection. / Holland, Brendan J.; Conlan, Xavier A.; Stevenson, Paul G.; Tye, Susannah J; Reker, Ashlie; Barnett, Neil W.; Adcock, Jacqui L.; Francis, Paul S.

In: Analytical and Bioanalytical Chemistry, Vol. 406, No. 23, 01.09.2014, p. 5669-5676.

Research output: Contribution to journalArticle

Holland, Brendan J. ; Conlan, Xavier A. ; Stevenson, Paul G. ; Tye, Susannah J ; Reker, Ashlie ; Barnett, Neil W. ; Adcock, Jacqui L. ; Francis, Paul S. / Determination of neurotransmitters and their metabolites using one- and two-dimensional liquid chromatography with acidic potassium permanganate chemiluminescence detection. In: Analytical and Bioanalytical Chemistry. 2014 ; Vol. 406, No. 23. pp. 5669-5676.
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