Determination of leucine flux in vivo by gas chromatography—mass spectrometry utilizing stable isotopes for trace and internal standard

Morey W. Haymond, Campbell P. Howard, John M. Miles, John E. Gerich

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A simple and reliable method is described for the determination of leucine flux in vivo using two stable isotopes of leucine and gas chromatography—mass spectrometry (GCMS). [6,6,6-2H3]Leucine is administered as a primed-dose constant infusion in vivo and dl-[2H7]leucine is added to plasma as an internal standard. Plasma leucine concentration and moles per cent enrichment of [2H3]leucine can be determined simultaneously by GCMS and selected ion monitoring. Leucine flux calculated from the [6,6,6-2H3]leucine data was nearly identical to that obtained with l-[U-14C]leucine in dogs. This method is readily applicable to the study of leucine metabolism in humans of all ages and laboratory animals.

Original languageEnglish (US)
Pages (from-to)403-409
Number of pages7
JournalJournal of Chromatography B: Biomedical Applications
Volume183
Issue number4
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Chemistry(all)

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