Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry

John M. Miles, W. Frederick Schwenk, Kelly L. McClean, Morey W. Haymond

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A simple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of [3,4-13C2]acetoacetate is described. Mole percent enrichment of β-[13C2]hydroxybutyrate and [13C2]acetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalAnalytical Biochemistry
Volume141
Issue number1
DOIs
StatePublished - Aug 15 1984

Fingerprint

Ketone Bodies
Isotopes
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Hydroxybutyrates
Impact ionization
Laboratory Animals
Metabolism
Animals
Dogs
Electrons
Ions
Fluxes
Monitoring
Experiments
acetoacetic acid

Keywords

  • gas chromatography/mass spectrometry
  • isotope dilution methods
  • ketone bodies
  • stable isotopes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry. / Miles, John M.; Schwenk, W. Frederick; McClean, Kelly L.; Haymond, Morey W.

In: Analytical Biochemistry, Vol. 141, No. 1, 15.08.1984, p. 110-115.

Research output: Contribution to journalArticle

Miles, John M. ; Schwenk, W. Frederick ; McClean, Kelly L. ; Haymond, Morey W. / Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry. In: Analytical Biochemistry. 1984 ; Vol. 141, No. 1. pp. 110-115.
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