Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry

John M. Miles; W. Frederick Schwenk; Kelly L. McClean; Morey W. Haymond

doi:10.1016/0003-2697(84)90432-9

Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry

John M. Miles, W. Frederick Schwenk, Kelly L. McClean, Morey W. Haymond

Endocrinology, Diabetes, Metabolism, and Nutrition

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A simple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of [3,4-¹³C₂]acetoacetate is described. Mole percent enrichment of β-[¹³C₂]hydroxybutyrate and [¹³C₂]acetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans.

Original language	English (US)
Pages (from-to)	110-115
Number of pages	6
Journal	Analytical Biochemistry
Volume	141
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(84)90432-9
State	Published - Aug 15 1984

Keywords

gas chromatography/mass spectrometry
isotope dilution methods
ketone bodies
stable isotopes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(84)90432-9

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title = "Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry",

abstract = "A simple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of [3,4-13C2]acetoacetate is described. Mole percent enrichment of β-[13C2]hydroxybutyrate and [13C2]acetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans.",

keywords = "gas chromatography/mass spectrometry, isotope dilution methods, ketone bodies, stable isotopes",

author = "Miles, {John M.} and Schwenk, {W. Frederick} and McClean, {Kelly L.} and Haymond, {Morey W.}",

note = "Funding Information: {\textquoteright} This work was supportedb y USPHS Grants AM26989 and AM204 11 and by the Wasie and Mayo Foundations. Dr. Schwenk was supported by USPHS Grant AM07352. z To whom reprint requests should be addressed.",

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T1 - Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry

AU - Miles, John M.

AU - Schwenk, W. Frederick

AU - McClean, Kelly L.

AU - Haymond, Morey W.

N1 - Funding Information: ’ This work was supportedb y USPHS Grants AM26989 and AM204 11 and by the Wasie and Mayo Foundations. Dr. Schwenk was supported by USPHS Grant AM07352. z To whom reprint requests should be addressed.

PY - 1984/8/15

Y1 - 1984/8/15

N2 - A simple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of [3,4-13C2]acetoacetate is described. Mole percent enrichment of β-[13C2]hydroxybutyrate and [13C2]acetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans.

AB - A simple and reliable method for the determination of ketone body turnover in vivo using a primed, continuous infusion of [3,4-13C2]acetoacetate is described. Mole percent enrichment of β-[13C2]hydroxybutyrate and [13C2]acetoacetate is determined by gas chromatography/mass spectrometry using electron-impact ionization and selected ion monitoring. Ketone body flux data are provided from preliminary dog experiments. The method is readily applicable to the study of ketone body metabolism in both laboratory animals and humans.

KW - gas chromatography/mass spectrometry

KW - isotope dilution methods

KW - ketone bodies

KW - stable isotopes

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Determination of ketone body turnover in vivo with stable isotopes, utilizing gas chromatography/mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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