Determination of gene and chromosome dosage in prostatic intraepithelial neoplasia and carcinoma

Junqi Qian, Robert B. Jenkins, David G. Bostwick

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

OBJECTIVE: To review the utility of fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR) and comparative genomic hybridization (CGH) in the determination of gene and chromosome dosage in cancer specimens and to discuss the genetic association between prostatic intraepithelial neoplasia (PIN) and adenocarcinoma as detected by these three techniques. STUDY DESIGN: FISH, PCR, and CGH were applied to the same specimens to clarify discrepancies observed by a single technique alone and to further understand prostate carcinogenesis. RESULTS: The most common genetic alterations in PIN and carcinoma were (1) gain of chromosome 7, particularly 7q31; (2) loss of Sp and gain of 8q; and (3) loss of 10q, 16q and 18q. CONCLUSION: Using different techniques on the same specimen was useful to determine genetic relationships between cancer and its precursors. PIN and prostatic carcinoma loci have a similar proportion of genetic changes, but loci of carcinoma usually have more alterations. This supports the hypothesis that PIN is the most likely precursor of prostatic carcinoma.

Original languageEnglish (US)
Pages (from-to)373-380
Number of pages8
JournalAnalytical and Quantitative Cytology and Histology
Volume20
Issue number5
StatePublished - Oct 1 1998

Keywords

  • CGH
  • Chromosome abnormality
  • FISH
  • Neoplasm metastasis
  • PCR
  • Prostatic intraepithelial neoplasia
  • Prostatic neoplasms

ASJC Scopus subject areas

  • Anatomy
  • Histology

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