Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis

Liu Yi; Ling Xiaomei; Fan Hui; Zhong Yingcheng; Li Dan; Wang Ying

doi:10.1016/j.chroma.2007.01.005

Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis

Liu Yi, Ling Xiaomei, Fan Hui, Zhong Yingcheng, Li Dan, Wang Ying

Cardiovascular Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between heparin and programmed cell death 5 (PDCD5) qualitatively and quantitatively. The binding constant of the interaction between PDCD5 and heparin calculated by Scatchard analysis was 4.17 × 10⁴ M^-1 and the binding sites located in the C-terminal region of PDCD5 (residues 109-115). The precisions of migration times, peak heights and binding constants, expressed as the relative standard deviation, were less than 2.4%, 1.1% and 5.7%, respectively.

Original language	English (US)
Pages (from-to)	284-287
Number of pages	4
Journal	Journal of Chromatography A
Volume	1143
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2007.01.005
State	Published - Mar 2 2007

Keywords

Capillary zone electrophoresis
Heparin
PDCD5

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2007.01.005

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title = "Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis",

abstract = "A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between heparin and programmed cell death 5 (PDCD5) qualitatively and quantitatively. The binding constant of the interaction between PDCD5 and heparin calculated by Scatchard analysis was 4.17 × 104 M-1 and the binding sites located in the C-terminal region of PDCD5 (residues 109-115). The precisions of migration times, peak heights and binding constants, expressed as the relative standard deviation, were less than 2.4%, 1.1% and 5.7%, respectively.",

keywords = "Capillary zone electrophoresis, Heparin, PDCD5",

author = "Liu Yi and Ling Xiaomei and Fan Hui and Zhong Yingcheng and Li Dan and Wang Ying",

note = "Funding Information: This work was financially supported by the National High-Tech Research and Development Program of China (863 Program) (2004AA2Z3783 and 2002BA711A01) and a grant from the State Key Program of Basic Research (G2000056909).",

year = "2007",

month = mar,

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doi = "10.1016/j.chroma.2007.01.005",

language = "English (US)",

volume = "1143",

pages = "284--287",

journal = "Journal of Chromatography",

issn = "0021-9673",

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number = "1-2",

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TY - JOUR

T1 - Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis

AU - Yi, Liu

AU - Xiaomei, Ling

AU - Hui, Fan

AU - Yingcheng, Zhong

AU - Dan, Li

AU - Ying, Wang

N1 - Funding Information: This work was financially supported by the National High-Tech Research and Development Program of China (863 Program) (2004AA2Z3783 and 2002BA711A01) and a grant from the State Key Program of Basic Research (G2000056909).

PY - 2007/3/2

Y1 - 2007/3/2

N2 - A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between heparin and programmed cell death 5 (PDCD5) qualitatively and quantitatively. The binding constant of the interaction between PDCD5 and heparin calculated by Scatchard analysis was 4.17 × 104 M-1 and the binding sites located in the C-terminal region of PDCD5 (residues 109-115). The precisions of migration times, peak heights and binding constants, expressed as the relative standard deviation, were less than 2.4%, 1.1% and 5.7%, respectively.

AB - A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between heparin and programmed cell death 5 (PDCD5) qualitatively and quantitatively. The binding constant of the interaction between PDCD5 and heparin calculated by Scatchard analysis was 4.17 × 104 M-1 and the binding sites located in the C-terminal region of PDCD5 (residues 109-115). The precisions of migration times, peak heights and binding constants, expressed as the relative standard deviation, were less than 2.4%, 1.1% and 5.7%, respectively.

KW - Capillary zone electrophoresis

KW - Heparin

KW - PDCD5

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U2 - 10.1016/j.chroma.2007.01.005

DO - 10.1016/j.chroma.2007.01.005

M3 - Article

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AN - SCOPUS:33846816388

SN - 0021-9673

VL - 1143

SP - 284

EP - 287

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 1-2

ER -

Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis

Abstract

Keywords

ASJC Scopus subject areas

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