Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves' ophthalmopathy and pretibial dermopathy

A. E. Heufelder, C. M. Dutton, G. Sarkar, K. A. Donovan, R. S. Bahn

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Fibroblasts are target cells for the autoimmune process in Graves' ophthalmopathy and pretibial dermopathy. Because the autoantigen involved in the hyperthyroidism of Graves' disease is the TSH receptor, we sought to determine whether RNA encoding this receptor might be present in retroocular and pretibial fibroblasts. RNA was reverse transcribed and the resulting cDNA was amplified by the polymerase chain reaction using primers spanning a region of the extracellular domain of the human TSH receptor. The predicted amplified product, verified by direct sequencing, was detected when RNA was derived from fibroblasts, but not from the nonfibroblast cells studied. The demonstration in fibroblasts of RNA encoding this important autoantigen in Graves' disease suggests that the TSH receptor might play a role in the pathogenesis of the connective tissue manifestations of this disease.

Original languageEnglish (US)
Pages (from-to)297-300
Number of pages4
JournalThyroid
Volume3
Issue number4
StatePublished - 1993

Fingerprint

Graves Ophthalmopathy
Thyrotropin Receptors
Fibroblasts
RNA
Connective Tissue Diseases
Graves Disease
Autoantigens
Hyperthyroidism
Complementary DNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Endocrinology

Cite this

Heufelder, A. E., Dutton, C. M., Sarkar, G., Donovan, K. A., & Bahn, R. S. (1993). Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves' ophthalmopathy and pretibial dermopathy. Thyroid, 3(4), 297-300.

Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves' ophthalmopathy and pretibial dermopathy. / Heufelder, A. E.; Dutton, C. M.; Sarkar, G.; Donovan, K. A.; Bahn, R. S.

In: Thyroid, Vol. 3, No. 4, 1993, p. 297-300.

Research output: Contribution to journalArticle

Heufelder, AE, Dutton, CM, Sarkar, G, Donovan, KA & Bahn, RS 1993, 'Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves' ophthalmopathy and pretibial dermopathy', Thyroid, vol. 3, no. 4, pp. 297-300.
Heufelder, A. E. ; Dutton, C. M. ; Sarkar, G. ; Donovan, K. A. ; Bahn, R. S. / Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves' ophthalmopathy and pretibial dermopathy. In: Thyroid. 1993 ; Vol. 3, No. 4. pp. 297-300.
@article{d64d924d1b4f47bfbccd6600319b60bf,
title = "Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves' ophthalmopathy and pretibial dermopathy",
abstract = "Fibroblasts are target cells for the autoimmune process in Graves' ophthalmopathy and pretibial dermopathy. Because the autoantigen involved in the hyperthyroidism of Graves' disease is the TSH receptor, we sought to determine whether RNA encoding this receptor might be present in retroocular and pretibial fibroblasts. RNA was reverse transcribed and the resulting cDNA was amplified by the polymerase chain reaction using primers spanning a region of the extracellular domain of the human TSH receptor. The predicted amplified product, verified by direct sequencing, was detected when RNA was derived from fibroblasts, but not from the nonfibroblast cells studied. The demonstration in fibroblasts of RNA encoding this important autoantigen in Graves' disease suggests that the TSH receptor might play a role in the pathogenesis of the connective tissue manifestations of this disease.",
author = "Heufelder, {A. E.} and Dutton, {C. M.} and G. Sarkar and Donovan, {K. A.} and Bahn, {R. S.}",
year = "1993",
language = "English (US)",
volume = "3",
pages = "297--300",
journal = "Thyroid",
issn = "1050-7256",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves' ophthalmopathy and pretibial dermopathy

AU - Heufelder, A. E.

AU - Dutton, C. M.

AU - Sarkar, G.

AU - Donovan, K. A.

AU - Bahn, R. S.

PY - 1993

Y1 - 1993

N2 - Fibroblasts are target cells for the autoimmune process in Graves' ophthalmopathy and pretibial dermopathy. Because the autoantigen involved in the hyperthyroidism of Graves' disease is the TSH receptor, we sought to determine whether RNA encoding this receptor might be present in retroocular and pretibial fibroblasts. RNA was reverse transcribed and the resulting cDNA was amplified by the polymerase chain reaction using primers spanning a region of the extracellular domain of the human TSH receptor. The predicted amplified product, verified by direct sequencing, was detected when RNA was derived from fibroblasts, but not from the nonfibroblast cells studied. The demonstration in fibroblasts of RNA encoding this important autoantigen in Graves' disease suggests that the TSH receptor might play a role in the pathogenesis of the connective tissue manifestations of this disease.

AB - Fibroblasts are target cells for the autoimmune process in Graves' ophthalmopathy and pretibial dermopathy. Because the autoantigen involved in the hyperthyroidism of Graves' disease is the TSH receptor, we sought to determine whether RNA encoding this receptor might be present in retroocular and pretibial fibroblasts. RNA was reverse transcribed and the resulting cDNA was amplified by the polymerase chain reaction using primers spanning a region of the extracellular domain of the human TSH receptor. The predicted amplified product, verified by direct sequencing, was detected when RNA was derived from fibroblasts, but not from the nonfibroblast cells studied. The demonstration in fibroblasts of RNA encoding this important autoantigen in Graves' disease suggests that the TSH receptor might play a role in the pathogenesis of the connective tissue manifestations of this disease.

UR - http://www.scopus.com/inward/record.url?scp=0027722302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027722302&partnerID=8YFLogxK

M3 - Article

C2 - 7509671

AN - SCOPUS:0027722302

VL - 3

SP - 297

EP - 300

JO - Thyroid

JF - Thyroid

SN - 1050-7256

IS - 4

ER -