Detection of trisomy 12 by FISH in untreated b -chronic lymphocytic leukemia: Correlation with stage and CD20 antigen expression intensity

Thomas Elmer Witzig, Thomas J. Borell, John F. Herath, Ayalew Tefferi, C. Y. Li, Robert Brian Jenkins

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21 Citations (Scopus)

Abstract

We studied cells from 30 controls and 85 cases of untreated B-chronic lymphocytic leukemia (CLL) with a fluorescence in-situ hybridization (FISH) technique utilizing a probe to chromosome 12. By use of a threshold of >2% for trisomy 12 for the CLL cases (the mean +3 SD for controls was 1.3% 20% (17/85) were trisomy 12. The mean % cells positive was 32.6 (median, 39.4; range, 2.4-79.1). There was a trend toward an higher incidence of trisomy 12 in patients with Rai stages 1-4 vs Rai 0 (p = 0.16). Forty-seven % (8/17) of patients with trisomy 12 had strong intensity CD20 antigen expression compared to 21% (14/68) of patients without trisomy 12 (p = 0.03). Trisomy 12 associated with CLL is easily detected by FISH with an overall incidence of 20% This technique should be applied to larger groups of patients to confirm the potential variation among Rai stages and immunophenotypic subgroups.

Original languageEnglish (US)
Pages (from-to)447-451
Number of pages5
JournalLeukemia and Lymphoma
Volume14
Issue number5-6
DOIs
StatePublished - 1994

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CD20 Antigens
Trisomy
B-Cell Chronic Lymphocytic Leukemia
Fluorescence In Situ Hybridization
Chromosomes, Human, Pair 12
Incidence

Keywords

  • Chronic lymphocytic leukemia
  • Cytogenetics

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

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title = "Detection of trisomy 12 by FISH in untreated b -chronic lymphocytic leukemia: Correlation with stage and CD20 antigen expression intensity",
abstract = "We studied cells from 30 controls and 85 cases of untreated B-chronic lymphocytic leukemia (CLL) with a fluorescence in-situ hybridization (FISH) technique utilizing a probe to chromosome 12. By use of a threshold of >2{\%} for trisomy 12 for the CLL cases (the mean +3 SD for controls was 1.3{\%} 20{\%} (17/85) were trisomy 12. The mean {\%} cells positive was 32.6 (median, 39.4; range, 2.4-79.1). There was a trend toward an higher incidence of trisomy 12 in patients with Rai stages 1-4 vs Rai 0 (p = 0.16). Forty-seven {\%} (8/17) of patients with trisomy 12 had strong intensity CD20 antigen expression compared to 21{\%} (14/68) of patients without trisomy 12 (p = 0.03). Trisomy 12 associated with CLL is easily detected by FISH with an overall incidence of 20{\%} This technique should be applied to larger groups of patients to confirm the potential variation among Rai stages and immunophenotypic subgroups.",
keywords = "Chronic lymphocytic leukemia, Cytogenetics",
author = "Witzig, {Thomas Elmer} and Borell, {Thomas J.} and Herath, {John F.} and Ayalew Tefferi and Li, {C. Y.} and Jenkins, {Robert Brian}",
year = "1994",
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T1 - Detection of trisomy 12 by FISH in untreated b -chronic lymphocytic leukemia

T2 - Correlation with stage and CD20 antigen expression intensity

AU - Witzig, Thomas Elmer

AU - Borell, Thomas J.

AU - Herath, John F.

AU - Tefferi, Ayalew

AU - Li, C. Y.

AU - Jenkins, Robert Brian

PY - 1994

Y1 - 1994

N2 - We studied cells from 30 controls and 85 cases of untreated B-chronic lymphocytic leukemia (CLL) with a fluorescence in-situ hybridization (FISH) technique utilizing a probe to chromosome 12. By use of a threshold of >2% for trisomy 12 for the CLL cases (the mean +3 SD for controls was 1.3% 20% (17/85) were trisomy 12. The mean % cells positive was 32.6 (median, 39.4; range, 2.4-79.1). There was a trend toward an higher incidence of trisomy 12 in patients with Rai stages 1-4 vs Rai 0 (p = 0.16). Forty-seven % (8/17) of patients with trisomy 12 had strong intensity CD20 antigen expression compared to 21% (14/68) of patients without trisomy 12 (p = 0.03). Trisomy 12 associated with CLL is easily detected by FISH with an overall incidence of 20% This technique should be applied to larger groups of patients to confirm the potential variation among Rai stages and immunophenotypic subgroups.

AB - We studied cells from 30 controls and 85 cases of untreated B-chronic lymphocytic leukemia (CLL) with a fluorescence in-situ hybridization (FISH) technique utilizing a probe to chromosome 12. By use of a threshold of >2% for trisomy 12 for the CLL cases (the mean +3 SD for controls was 1.3% 20% (17/85) were trisomy 12. The mean % cells positive was 32.6 (median, 39.4; range, 2.4-79.1). There was a trend toward an higher incidence of trisomy 12 in patients with Rai stages 1-4 vs Rai 0 (p = 0.16). Forty-seven % (8/17) of patients with trisomy 12 had strong intensity CD20 antigen expression compared to 21% (14/68) of patients without trisomy 12 (p = 0.03). Trisomy 12 associated with CLL is easily detected by FISH with an overall incidence of 20% This technique should be applied to larger groups of patients to confirm the potential variation among Rai stages and immunophenotypic subgroups.

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