Detection of trisomy 12 by FISH in untreated b -chronic lymphocytic leukemia: Correlation with stage and CD20 antigen expression intensity

Thomas E. Witzig, Thomas J. Borell, John F. Herath, Ayalew Tefferi, C. Y. Li, Robert B. Jenkins

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Abstract

We studied cells from 30 controls and 85 cases of untreated B-chronic lymphocytic leukemia (CLL) with a fluorescence in-situ hybridization (FISH) technique utilizing a probe to chromosome 12. By use of a threshold of >2% for trisomy 12 for the CLL cases (the mean +3 SD for controls was 1.3% 20% (17/85) were trisomy 12. The mean % cells positive was 32.6 (median, 39.4; range, 2.4-79.1). There was a trend toward an higher incidence of trisomy 12 in patients with Rai stages 1-4 vs Rai 0 (p = 0.16). Forty-seven % (8/17) of patients with trisomy 12 had strong intensity CD20 antigen expression compared to 21% (14/68) of patients without trisomy 12 (p = 0.03). Trisomy 12 associated with CLL is easily detected by FISH with an overall incidence of 20% This technique should be applied to larger groups of patients to confirm the potential variation among Rai stages and immunophenotypic subgroups.

Original languageEnglish (US)
Pages (from-to)447-451
Number of pages5
JournalLeukemia and Lymphoma
Volume14
Issue number5-6
DOIs
StatePublished - Jan 1 1994

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Keywords

  • Chronic lymphocytic leukemia
  • Cytogenetics

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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