Detection of Sarcocystis parasites in retail beef: A regional survey combining histological and genetic detection methods

Bobbi Pritt, Thomas Trainer, Linda Simmons-Arnold, Mark Evans, Detiger Dunams, Benjamin M. Rosenthal

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Sarcocystis spp. are parasitic protists acquired when undercooked, cyst-laden meat is consumed. While both Sarcocystis hominis and S. cruzi encyst in beef, only S. hominis is pathogenic to humans. In this study, we used histological methods and novel molecular techniques to determine the regional prevalence and identity of Sarcocystis spp. in retail beef. Of 110 samples, 60 supported amplification of parasite rRNA by PCR. All 41 sequenced representatives were identified as S. cruzi. To compare detection methods, 48 samples were then examined in parallel by histology and PCR, and 16 and 26 samples, respectively, were positive. Five samples positive by initial histologic sections were not amplified by PCR. Fifteen PCR-positive samples did not contain sarcocysts on initial histologic section, but additional sections from these samples revealed sarcocysts in an additional 12 samples. When combined, histology with additional sections and PCR detected 31 positive specimens of the 48 total specimens. We found no evidence of human pathogen S. hominis and confirm that cattle pathogen S. cruzi is highly prevalent in this regional sample. PCR assays may increase the detection sensitivity of Sarcocystis spp. and contribute diagnostic precision.

Original languageEnglish (US)
Pages (from-to)2144-2147
Number of pages4
JournalJournal of Food Protection
Volume71
Issue number10
StatePublished - Oct 2008

Fingerprint

Sarcocystis
Parasites
beef
parasites
Polymerase Chain Reaction
sarcocysts
sampling
Histology
methodology
histology
Sarcocystis cruzi
Meat
Surveys and Questionnaires
Red Meat
Cysts
pathogens
ribosomal RNA
meat
cattle

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Pritt, B., Trainer, T., Simmons-Arnold, L., Evans, M., Dunams, D., & Rosenthal, B. M. (2008). Detection of Sarcocystis parasites in retail beef: A regional survey combining histological and genetic detection methods. Journal of Food Protection, 71(10), 2144-2147.

Detection of Sarcocystis parasites in retail beef : A regional survey combining histological and genetic detection methods. / Pritt, Bobbi; Trainer, Thomas; Simmons-Arnold, Linda; Evans, Mark; Dunams, Detiger; Rosenthal, Benjamin M.

In: Journal of Food Protection, Vol. 71, No. 10, 10.2008, p. 2144-2147.

Research output: Contribution to journalArticle

Pritt, B, Trainer, T, Simmons-Arnold, L, Evans, M, Dunams, D & Rosenthal, BM 2008, 'Detection of Sarcocystis parasites in retail beef: A regional survey combining histological and genetic detection methods', Journal of Food Protection, vol. 71, no. 10, pp. 2144-2147.
Pritt B, Trainer T, Simmons-Arnold L, Evans M, Dunams D, Rosenthal BM. Detection of Sarcocystis parasites in retail beef: A regional survey combining histological and genetic detection methods. Journal of Food Protection. 2008 Oct;71(10):2144-2147.
Pritt, Bobbi ; Trainer, Thomas ; Simmons-Arnold, Linda ; Evans, Mark ; Dunams, Detiger ; Rosenthal, Benjamin M. / Detection of Sarcocystis parasites in retail beef : A regional survey combining histological and genetic detection methods. In: Journal of Food Protection. 2008 ; Vol. 71, No. 10. pp. 2144-2147.
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