TY - JOUR
T1 - Detection of Progressive Myocardial Tissue Injury by Ultrasonic Integrated Backscatter Immediately After Coronary Reperfusion
AU - Katayama, Minako
AU - Jiamsripong, Panupong
AU - McMahon, Eileen M.
AU - Lombari, Theresa R.
AU - Bukatina, Anna E.
AU - Wu, Qing
AU - Marler, Ronald J.
AU - Belohlavek, Marek
N1 - Funding Information:
The study was supported by the American Heart Association Grant-in-Aid 2060631 to Dr. Belohlavek. We thank Dr. Naomi Gades and Adriana Orozco for veterinary help, Jacquelyn Lombari for her assistance with the experiments during her internship in our laboratory, and Danielle Wright for secretarial help.
PY - 2012/9
Y1 - 2012/9
N2 - Myocardial reperfusion following ischemia may paradoxically cause additional injury, including microvascular damage and edema. These structural alterations augment tissue echogenicity, which is measurable by ultrasonic integrated backscatter (IB). We sought to characterize alterations in myocardial IB in an ischemic and reperfused region of the rat heart. Myocardial IB of the regions of interest in 12 adult male Sprague-Dawley rats was studied at baseline, during ischemia, and chronologically after coronary reopening, using an ultrasound frequency of 8 MHz. IB did not significantly change between baseline and ischemia. However, within 1 min of reperfusion, IB significantly increased and continued to increase until 10 min of reperfusion, when a plateau was reached. Areas of high echogenicity were comparable to infarcted areas on gross pathologic slices and had edema with extravasation of red blood cells. Myocardial reperfusion following ischemia significantly augments tissue echogenicity. A continuing increase of IB suggests a rapid progression of reperfusion injury.
AB - Myocardial reperfusion following ischemia may paradoxically cause additional injury, including microvascular damage and edema. These structural alterations augment tissue echogenicity, which is measurable by ultrasonic integrated backscatter (IB). We sought to characterize alterations in myocardial IB in an ischemic and reperfused region of the rat heart. Myocardial IB of the regions of interest in 12 adult male Sprague-Dawley rats was studied at baseline, during ischemia, and chronologically after coronary reopening, using an ultrasound frequency of 8 MHz. IB did not significantly change between baseline and ischemia. However, within 1 min of reperfusion, IB significantly increased and continued to increase until 10 min of reperfusion, when a plateau was reached. Areas of high echogenicity were comparable to infarcted areas on gross pathologic slices and had edema with extravasation of red blood cells. Myocardial reperfusion following ischemia significantly augments tissue echogenicity. A continuing increase of IB suggests a rapid progression of reperfusion injury.
KW - Acute myocardial infarction
KW - Integrated backscatter
KW - Myocardial edema
KW - Reperfusion injury
KW - Ultrasound tissue characterization
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U2 - 10.1016/j.ultrasmedbio.2012.03.002
DO - 10.1016/j.ultrasmedbio.2012.03.002
M3 - Article
C2 - 22763007
AN - SCOPUS:84864581891
SN - 0301-5629
VL - 38
SP - 1662
EP - 1669
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 9
ER -