Detection of peptide nucleic acids in tissue extracts of treated animals by gel mobility shift assay

Karen Jansen, Elliott Richelson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We have developed a sensitive and reproducible gel mobility shift assay to detect PNA oligomers in tissue of treated animals. PNA present in purified tissue extracts of treated animals is hybridized to a 33P-labelled DNA oligomer probe, and analyzed by polyacrylamide gel electrophoresis. The PNA-DNA hybrid migrates more slowly than the DNA probe alone and can be quantified relative to a standard curve. This detection method is useful for detecting PNAs in many different tissues, including brain, heart, kidney, liver, spleen, and serum, as well as cells in culture. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)31-34
Number of pages4
JournalJournal of Biochemical and Biophysical Methods
Volume42
Issue number1-2
DOIs
StatePublished - Jan 3 2000

Keywords

  • Antigene
  • Antisense
  • Brain

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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