Detection of macromolecular inversion–induced structural changes in osteosarcoma cells by FTIR microspectroscopy

Vitalii Serdiuk, Kristen L. Shogren, Tetiana Kovalenko, Bakhtiyor Rasulev, Michael Yaszemski, Avudaiappan Maran, Andriy Voronov

Research output: Contribution to journalArticlepeer-review

Abstract

Fourier transform infrared (FTIR) microspectroscopy provides a biochemical fingerprint of the cells. In this study, chemical changes in 143B osteosarcoma cells were investigated using FTIR analysis of cancer cells after their treatment with polymeric invertible micellar assemblies (IMAs) and curcumin-loaded IMAs and compared with untreated osteosarcoma cells. A comprehensive principal component analysis (PCA) was applied to analyze the FTIR results and confirm noticeable changes in cell surface chemical structures in the fingerprint regions of 1480–900 cm−1. The performed clustering shows visible differences for all investigated groups of cancer cells. It is demonstrated that a combination of FTIR microspectroscopy with PCA can be an efficient approach in determining interactions of osteosarcoma cells and drug-loaded polymer micellar assemblies. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)7253-7262
Number of pages10
JournalAnalytical and bioanalytical chemistry
Volume412
Issue number26
DOIs
StatePublished - Oct 1 2020

Keywords

  • Analysis
  • Cancer cells
  • Classification
  • FTIR microspectroscopy
  • PCA
  • Polymeric assembly

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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