Detection of macromolecular inversion–induced structural changes in osteosarcoma cells by FTIR microspectroscopy

Vitalii Serdiuk; Kristen L. Shogren; Tetiana Kovalenko; Bakhtiyor Rasulev; Michael Yaszemski; Avudaiappan Maran; Andriy Voronov

doi:10.1007/s00216-020-02858-4

Detection of macromolecular inversion–induced structural changes in osteosarcoma cells by FTIR microspectroscopy

Vitalii Serdiuk, Kristen L. Shogren, Tetiana Kovalenko, Bakhtiyor Rasulev, Michael Yaszemski, Avudaiappan Maran, Andriy Voronov

Orthopedic Surgery

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Fourier transform infrared (FTIR) microspectroscopy provides a biochemical fingerprint of the cells. In this study, chemical changes in 143B osteosarcoma cells were investigated using FTIR analysis of cancer cells after their treatment with polymeric invertible micellar assemblies (IMAs) and curcumin-loaded IMAs and compared with untreated osteosarcoma cells. A comprehensive principal component analysis (PCA) was applied to analyze the FTIR results and confirm noticeable changes in cell surface chemical structures in the fingerprint regions of 1480–900 cm⁻¹. The performed clustering shows visible differences for all investigated groups of cancer cells. It is demonstrated that a combination of FTIR microspectroscopy with PCA can be an efficient approach in determining interactions of osteosarcoma cells and drug-loaded polymer micellar assemblies. [Figure not available: see fulltext.]

Original language	English (US)
Pages (from-to)	7253-7262
Number of pages	10
Journal	Analytical and bioanalytical chemistry
Volume	412
Issue number	26
DOIs	https://doi.org/10.1007/s00216-020-02858-4
State	Published - Oct 1 2020

Keywords

Analysis
Cancer cells
Classification
FTIR microspectroscopy
PCA
Polymeric assembly

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1007/s00216-020-02858-4

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title = "Detection of macromolecular inversion–induced structural changes in osteosarcoma cells by FTIR microspectroscopy",

abstract = "Fourier transform infrared (FTIR) microspectroscopy provides a biochemical fingerprint of the cells. In this study, chemical changes in 143B osteosarcoma cells were investigated using FTIR analysis of cancer cells after their treatment with polymeric invertible micellar assemblies (IMAs) and curcumin-loaded IMAs and compared with untreated osteosarcoma cells. A comprehensive principal component analysis (PCA) was applied to analyze the FTIR results and confirm noticeable changes in cell surface chemical structures in the fingerprint regions of 1480–900 cm−1. The performed clustering shows visible differences for all investigated groups of cancer cells. It is demonstrated that a combination of FTIR microspectroscopy with PCA can be an efficient approach in determining interactions of osteosarcoma cells and drug-loaded polymer micellar assemblies. [Figure not available: see fulltext.]",

keywords = "Analysis, Cancer cells, Classification, FTIR microspectroscopy, PCA, Polymeric assembly",

author = "Vitalii Serdiuk and Shogren, {Kristen L.} and Tetiana Kovalenko and Bakhtiyor Rasulev and Michael Yaszemski and Avudaiappan Maran and Andriy Voronov",

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doi = "10.1007/s00216-020-02858-4",

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AU - Serdiuk, Vitalii

AU - Shogren, Kristen L.

AU - Kovalenko, Tetiana

AU - Rasulev, Bakhtiyor

AU - Yaszemski, Michael

AU - Maran, Avudaiappan

AU - Voronov, Andriy

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Fourier transform infrared (FTIR) microspectroscopy provides a biochemical fingerprint of the cells. In this study, chemical changes in 143B osteosarcoma cells were investigated using FTIR analysis of cancer cells after their treatment with polymeric invertible micellar assemblies (IMAs) and curcumin-loaded IMAs and compared with untreated osteosarcoma cells. A comprehensive principal component analysis (PCA) was applied to analyze the FTIR results and confirm noticeable changes in cell surface chemical structures in the fingerprint regions of 1480–900 cm−1. The performed clustering shows visible differences for all investigated groups of cancer cells. It is demonstrated that a combination of FTIR microspectroscopy with PCA can be an efficient approach in determining interactions of osteosarcoma cells and drug-loaded polymer micellar assemblies. [Figure not available: see fulltext.]

AB - Fourier transform infrared (FTIR) microspectroscopy provides a biochemical fingerprint of the cells. In this study, chemical changes in 143B osteosarcoma cells were investigated using FTIR analysis of cancer cells after their treatment with polymeric invertible micellar assemblies (IMAs) and curcumin-loaded IMAs and compared with untreated osteosarcoma cells. A comprehensive principal component analysis (PCA) was applied to analyze the FTIR results and confirm noticeable changes in cell surface chemical structures in the fingerprint regions of 1480–900 cm−1. The performed clustering shows visible differences for all investigated groups of cancer cells. It is demonstrated that a combination of FTIR microspectroscopy with PCA can be an efficient approach in determining interactions of osteosarcoma cells and drug-loaded polymer micellar assemblies. [Figure not available: see fulltext.]

KW - Analysis

KW - Cancer cells

KW - Classification

KW - FTIR microspectroscopy

KW - PCA

KW - Polymeric assembly

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Detection of macromolecular inversion–induced structural changes in osteosarcoma cells by FTIR microspectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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