Detection of lipopolysaccharides in serum using a waveguide-based optical biosensor

Aneesa Noormohamed, Loreen R. Stromberg, Aaron S. Anderson, Zachary Karim, Priya Dighe, Prakasha Kempaiah, John M. Ong'echa, Douglas J. Perkins, Norman Doggett, Benjamin McMahon, Harshini Mukundan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Direct ultra-sensitive detection of pathogen biomarkers in blood could provide a universal strategy for diagnosis of bacterial infections, which remain a leading cause of morbidity and mortality in many areas of the world. Many factors complicate diagnosis, including the presence of multiple co-infections in a given patient, and lack of infrastructure in rural settings. In some pediatric patients, such as those in areas with poor resources, an additional challenge exists with low sample volumes due to age and other health factors such as anemia and dehydration. Our team is working on developing novel diagnostic assays, with a waveguide-based biosensor platform, to rapidly and specifically identify pathogen biomarkers from small samples of serum or plasma, allowing for the timely and sensitive diagnosis of infection at the point of care. In addition to the platform, we have developed novel membrane insertion and lipoprotein capture assay methods, to capture lipidated pathogen biomarkers in aqueous blood, by virtue of their interactions with host lipoprotein carriers. Herein, we demonstrate our efforts to adapt the lipoprotein capture assay for the detection of small concentrations of pathogen-secreted lipopolysaccharides in aqueous blood, with the ultimate aim of diagnosing Gram-negative infections effectively.

Original languageEnglish (US)
Title of host publicationOptical Diagnostics and Sensing XVII
Subtitle of host publicationToward Point-of-Care Diagnostics
EditorsGerard L. Cote
PublisherSPIE
ISBN (Electronic)9781510605855
DOIs
StatePublished - 2017
EventOptical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics - San Francisco, United States
Duration: Jan 30 2017Jan 31 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10072
ISSN (Print)1605-7422

Conference

ConferenceOptical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics
Country/TerritoryUnited States
CitySan Francisco
Period1/30/171/31/17

Keywords

  • Lipid bilayers
  • Lipopolysaccharides (LPS)
  • Lipoprotein capture
  • Lipoproteins
  • Waveguide biosensor

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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