Detection of hypodiploidy using multi-parameter flow cytometric analysis

A prognostic indicator in multiple myeloma

R. J. Morgan, N. J. Gonchoroff, J. A. Katzmann, Thomas Elmer Witzig, R. A. Kyle, P. R. Greipp

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We analyzed 49 patients with multiple myeloma (MM) using propidium iodide (PI) staining and flow cytometry to assess DNA content of MM cells in bone marrow. In 12 patients the presence or absence of DNA content aneuploidy could not be determined by analysis of single-parameter DNA histograms alone. Using forward light scatter measurement, resolution of ploidy was readily obtained in all but three cases. Assuming the DNA content of the low light scatter population to be diploid, we were able to classify 7 cases as hypodiploid and 39 as hyperdiploid or diploid. Hyperdiploid or diploid patients survived a median of 24 months and hypodiploid patients only 2.5 months. We conclude that multiparameter analysis of DNA content and light scatter provides easier analysis for the presence or absence of aneuploidy. Patients with DNA content hypodiploidy have a short survival.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalAmerican Journal of Hematology
Volume30
Issue number4
StatePublished - 1989

Fingerprint

Multiple Myeloma
DNA
Diploidy
Polyploidy
Aneuploidy
Light
Propidium
Ploidies
Bone Marrow Cells
Flow Cytometry
Staining and Labeling
Survival
Population

ASJC Scopus subject areas

  • Hematology

Cite this

Morgan, R. J., Gonchoroff, N. J., Katzmann, J. A., Witzig, T. E., Kyle, R. A., & Greipp, P. R. (1989). Detection of hypodiploidy using multi-parameter flow cytometric analysis: A prognostic indicator in multiple myeloma. American Journal of Hematology, 30(4), 195-200.

Detection of hypodiploidy using multi-parameter flow cytometric analysis : A prognostic indicator in multiple myeloma. / Morgan, R. J.; Gonchoroff, N. J.; Katzmann, J. A.; Witzig, Thomas Elmer; Kyle, R. A.; Greipp, P. R.

In: American Journal of Hematology, Vol. 30, No. 4, 1989, p. 195-200.

Research output: Contribution to journalArticle

Morgan, R. J. ; Gonchoroff, N. J. ; Katzmann, J. A. ; Witzig, Thomas Elmer ; Kyle, R. A. ; Greipp, P. R. / Detection of hypodiploidy using multi-parameter flow cytometric analysis : A prognostic indicator in multiple myeloma. In: American Journal of Hematology. 1989 ; Vol. 30, No. 4. pp. 195-200.
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