Detection of hybrid (combinatorial) Ia antigens using parent anti-F1 sera

S. Yokota, W. P. Lafuse, J. F. McCormick, C. S. David

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4 Citations (Scopus)

Abstract

Ia specificities 22 and 23 were initially identified by using conventional alloantisera and were mapped to the I-E subregion of k and d haplotypes on the basis of their reactivity with selected recombinants. Recently we found that Ia 22 and Ia 23 are hybrid determinants on the basis of their expression on selected F1 cells, but absence from both parental cells. Initial attempts to detect hybrid Ia antigens by immunizing parents with F1 cells were unsucessful. By utilizing lipopolysaccharide (LPS)-stimulated F1 spleen lymphoblasts as immunogens, 1 of the parents as recipient and the other parent cells for absorption of antisera, specific anti-Ia.22 and 23 antibodies were produced. The specificity of these parent anti-F1 sera was confirmed by cytotoxic and immunoprecipitation analyses.

Original languageEnglish (US)
Pages (from-to)371-374
Number of pages4
JournalJournal of Immunology
Volume126
Issue number1
StatePublished - 1981

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Histocompatibility Antigens Class II
Serum
Immunoprecipitation
Haplotypes
Lipopolysaccharides
Immune Sera
Spleen
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Yokota, S., Lafuse, W. P., McCormick, J. F., & David, C. S. (1981). Detection of hybrid (combinatorial) Ia antigens using parent anti-F1 sera. Journal of Immunology, 126(1), 371-374.

Detection of hybrid (combinatorial) Ia antigens using parent anti-F1 sera. / Yokota, S.; Lafuse, W. P.; McCormick, J. F.; David, C. S.

In: Journal of Immunology, Vol. 126, No. 1, 1981, p. 371-374.

Research output: Contribution to journalArticle

Yokota, S, Lafuse, WP, McCormick, JF & David, CS 1981, 'Detection of hybrid (combinatorial) Ia antigens using parent anti-F1 sera', Journal of Immunology, vol. 126, no. 1, pp. 371-374.
Yokota S, Lafuse WP, McCormick JF, David CS. Detection of hybrid (combinatorial) Ia antigens using parent anti-F1 sera. Journal of Immunology. 1981;126(1):371-374.
Yokota, S. ; Lafuse, W. P. ; McCormick, J. F. ; David, C. S. / Detection of hybrid (combinatorial) Ia antigens using parent anti-F1 sera. In: Journal of Immunology. 1981 ; Vol. 126, No. 1. pp. 371-374.
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