Detection of human papilloma virus and p16 expression in high-grade adenoid cystic carcinoma of the head and neck

Jennifer M. Boland, Ellen McPhail, Joaquín J. García, Jean E. Lewis, David J. Schembri-Wismayer

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Squamous cell carcinoma of the head and neck, particularly basaloid squamous cell carcinoma, may be difficult to distinguish from high-grade adenoid cystic carcinoma. Evidence of human papilloma virus (HPV) infection, particularly HPV 16, is frequently found in non-keratinizing oropharyngeal squamous cell carcinoma. Immunoreactivity for p16, a surrogate marker for HPV infection, often parallels the HPV infection status in oropharyngeal squamous cell carcinoma. However, the incidence and correlation between p16 expression and HPV infection in high-grade adenoid cystic carcinoma is unknown. Sixteen cases of high-grade adenoid cystic carcinoma, three cases of dedifferentiated adenoid cystic carcinoma and eight cases of low-/intermediate-grade adenoid cystic carcinoma were identified for inclusion in the study. All cases were tested by immunohistochemistry for p16 expression and in situ hybridization for high-and low-risk HPV. Eight cases (100%) of low-to-intermediate-grade adenoid cystic carcinoma were focally positive for p16, all of which were negative for HPV. In all, 14 of 16 cases (88%) of high-grade adenoid cystic carcinoma and three cases (100%) of dedifferentiated adenoid cystic carcinoma were positive for p16; strong and diffuse staining was noted in three cases (3 of 19, 16%). Two cases (11%) of high-grade adenoid cystic carcinoma, which were also diffusely positive for p16, showed the presence of high-risk HPV. These findings suggest that the presence of HPV infection in high-grade adenoid cystic carcinoma is infrequent, even in the presence of p16 immunostaining. Nevertheless, HPV positivity should not be used to exclude the possibility of high-grade adenoid cystic carcinoma when the differential diagnosis includes squamous cell carcinoma. Moreover, although p16 overexpression is often used as a surrogate marker for HPV in squamous cell carcinoma, it cannot be used in this manner in high-grade adenoid cystic carcinoma.

Original languageEnglish (US)
Pages (from-to)529-536
Number of pages8
JournalModern Pathology
Volume25
Issue number4
DOIs
StatePublished - Apr 2012

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Papillomaviridae
Adenoid Cystic Carcinoma
Neck
Head
Virus Diseases
Squamous Cell Carcinoma
Biomarkers

Keywords

  • adenoid cystic carcinoma
  • HPV
  • p16

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Detection of human papilloma virus and p16 expression in high-grade adenoid cystic carcinoma of the head and neck. / Boland, Jennifer M.; McPhail, Ellen; García, Joaquín J.; Lewis, Jean E.; Schembri-Wismayer, David J.

In: Modern Pathology, Vol. 25, No. 4, 04.2012, p. 529-536.

Research output: Contribution to journalArticle

Boland, Jennifer M. ; McPhail, Ellen ; García, Joaquín J. ; Lewis, Jean E. ; Schembri-Wismayer, David J. / Detection of human papilloma virus and p16 expression in high-grade adenoid cystic carcinoma of the head and neck. In: Modern Pathology. 2012 ; Vol. 25, No. 4. pp. 529-536.
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