Detection of hantaviral antibodies among patients with hepatitis of unknown etiology in japan

Hiroaki Kariwa, Kumiko Yoshimatsu, Koichi Araki, Kazuaki Chayama, Hiromitsu Kumada, Michiko Ogino, Hideki Ebihara, Michael E. Murphy, Tetsuya Mizutani, Ikuo Takashima, Jiro Arikawa

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Hantaviral antibodies were detected in the sera from patients with hepatic disease of unknown etiology in Japan by several different serological diagnostic methods. A total of 105 sera from diseased patients which were negative to A-G hepatitis virus infections in the Tokyo area were tested. Among them, 3 out of 73 sera from patients with chronic hepatic disease were positive to hantaviral antibody by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescent antibody assay (IFA) and Western blot analysis (WB). Neutralizing antibody titers of the 3 sera to Seoul virus (SEO) were 4 to 8 times higher than those to Hantaan virus (HTN). However, all of the 32 sera from patients with acute hepatitis were negative for hantaviral antibody. Among the 60 patients with chronic hepatitis in Hokkaido which were serologically negative to B and C hepatitis virus infection, one was positive for hantaviral antibody by ELISA and WB. In contrast, the sera from healthy adults in Japan, 550 from the Honshu and Kyushu regions, and 1,000 from the Hokkaido region, were negative for hantavirus antibody. These results show that hantaviral antibodies are more frequently detected in patients with hepatic disease than in healthy adults. However, the observation that no positive sera were detected from patients with acute hepatitis implies that hantavirus might not be directly related to hepatitis.

Original languageEnglish (US)
Pages (from-to)357-362
Number of pages6
JournalMicrobiology and Immunology
Issue number5
StatePublished - 2000


  • Hantavirus
  • Hantavirus pulmonary syndrome
  • Hemorrhagic fever with renal syndrome
  • Hepatitis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology


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