Detection of epithelial ovarian cancer using 1H-NMR-based metabonomics

Kunle Odunsi, Robert M. Wollman, Christine B. Ambrosone, Alan Hutson, Susan E. McCann, Jonathan Tammela, John P. Geisler, Gregory Miller, Thomas Sellers, William Arthur Cliby, Feng Qian, Bernadette Keitz, Marilyn Intengan, Shashikant Lele, James L. Alderfer

Research output: Contribution to journalArticle

275 Citations (Scopus)

Abstract

Currently available serum biomarkers are insufficiently reliable to distinguish patients with epithelial ovarian cancer (EOC) from healthy individuals. Metabonomics, the study of metabolic processes in biologic systems, is based on the use of 1H-NMR spectroscopy and multivariate statistics for biochemical data generation and interpretation and may provide a characteristic fingerprint in disease. In an effort to examine the utility of the metabonomic approach for discriminating sera from women with EOC from healthy controls, we performed 1H-NMR spectroscopic analysis on preoperative serum specimens obtained from 38 patients with EOC, 12 patients with benign ovarian cysts and 53 healthy women. After data reduction, we applied both unsupervised Principal Component Analysis (PCA) and supervised Soft Independent Modeling of Class Analogy (SIMCA) for pattern recognition. The sensitivity and specificity tradeoffs were summarized for each variable using the area under the receiver-operating characteristic (ROC) curve. In addition, we analyzed the regions of NMR spectra that most strongly influence separation of sera of EOC patients from healthy controls. PCA analysis allowed correct separation of all serum specimens from 38 patients with EOC (100%) from all of the 21 premenopausal normal samples (100%) and from all the sera from patients with benign ovarian disease (100%). In addition, it was possible to correctly separate 37 of 38 (97.4%) cancer specimens from 31 of 32 (97%) postmenopausal control sera. SIMCA analysis using the Cooman's plot demonstrated that sera classes from patients with EOC, benign ovarian cysts and the postmenopausal healthy controls did not share multivariate space, providing validation for the class separation. ROC analysis indicated that the sera from patients with and without disease could be identified with 100% sensitivity and specificity at the 1H-NMR regions 2.77 parts per million (ppm) and 2.04 ppm from the origin (AUC of ROC curve = 1.0). In addition, the regression coefficients most influential for the EOC samples compared to postmenopausal controls lie around 83.7 ppm (due mainly to sugar hydrogens). Other loadings most influential for the EOC samples lie around 82.25 ppm and 81.18 ppm. These findings indicate that 1H-NMR metabonomic analysis of serum achieves complete separation of EOC patients from healthy controls. The metabonomic approach deserves further evaluation as a potential novel strategy for the early detection of epithelial ovarian cancer.

Original languageEnglish (US)
Pages (from-to)782-788
Number of pages7
JournalInternational Journal of Cancer
Volume113
Issue number5
DOIs
StatePublished - Feb 20 2005

Fingerprint

Metabolomics
Serum
ROC Curve
Ovarian Cysts
Principal Component Analysis
Ovarian epithelial cancer
Proton Magnetic Resonance Spectroscopy
Ovarian Diseases
Sensitivity and Specificity
Dermatoglyphics
Area Under Curve
Hydrogen
Magnetic Resonance Spectroscopy
Biomarkers

Keywords

  • H-NMR spectroscopy
  • Early diagnosis
  • Metabonomics
  • Ovarian cancer
  • Pattern recognition

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Odunsi, K., Wollman, R. M., Ambrosone, C. B., Hutson, A., McCann, S. E., Tammela, J., ... Alderfer, J. L. (2005). Detection of epithelial ovarian cancer using 1H-NMR-based metabonomics. International Journal of Cancer, 113(5), 782-788. https://doi.org/10.1002/ijc.20651

Detection of epithelial ovarian cancer using 1H-NMR-based metabonomics. / Odunsi, Kunle; Wollman, Robert M.; Ambrosone, Christine B.; Hutson, Alan; McCann, Susan E.; Tammela, Jonathan; Geisler, John P.; Miller, Gregory; Sellers, Thomas; Cliby, William Arthur; Qian, Feng; Keitz, Bernadette; Intengan, Marilyn; Lele, Shashikant; Alderfer, James L.

In: International Journal of Cancer, Vol. 113, No. 5, 20.02.2005, p. 782-788.

Research output: Contribution to journalArticle

Odunsi, K, Wollman, RM, Ambrosone, CB, Hutson, A, McCann, SE, Tammela, J, Geisler, JP, Miller, G, Sellers, T, Cliby, WA, Qian, F, Keitz, B, Intengan, M, Lele, S & Alderfer, JL 2005, 'Detection of epithelial ovarian cancer using 1H-NMR-based metabonomics', International Journal of Cancer, vol. 113, no. 5, pp. 782-788. https://doi.org/10.1002/ijc.20651
Odunsi K, Wollman RM, Ambrosone CB, Hutson A, McCann SE, Tammela J et al. Detection of epithelial ovarian cancer using 1H-NMR-based metabonomics. International Journal of Cancer. 2005 Feb 20;113(5):782-788. https://doi.org/10.1002/ijc.20651
Odunsi, Kunle ; Wollman, Robert M. ; Ambrosone, Christine B. ; Hutson, Alan ; McCann, Susan E. ; Tammela, Jonathan ; Geisler, John P. ; Miller, Gregory ; Sellers, Thomas ; Cliby, William Arthur ; Qian, Feng ; Keitz, Bernadette ; Intengan, Marilyn ; Lele, Shashikant ; Alderfer, James L. / Detection of epithelial ovarian cancer using 1H-NMR-based metabonomics. In: International Journal of Cancer. 2005 ; Vol. 113, No. 5. pp. 782-788.
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