Detection of diagnostically critical, often hidden, anomalies in complex karyotypes of haematological disorders using multicolour fluorescence in situ hybridization

S. M. Jalal, M. E. Law, J. Stamberg, R. Fonseca, J. R. Seely, W. H. Myers, C. A. Hanson

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Abstract

Multicolour fluorescence in situ hybridization (M-FISH) simultaneously detects all 24 human chromosomes in unique fluorescent colours. The identification of diagnostically critical gene rearrangement(s) in complex karyotypes of haematological disorders continues to be a challenge. We present five cases in which t(9;11), complex t(8;22), t(12;21) and t(11;14) were detected primarily using M-FISH and were confirmed using locus-specific probes. We conclude that M-FISH can be effective in complete characterization of critical gene rearrangements in haematological disorders.

Original languageEnglish (US)
Pages (from-to)975-980
Number of pages6
JournalBritish journal of haematology
Volume112
Issue number4
DOIs
StatePublished - Apr 30 2001

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Keywords

  • Complex karyotype
  • Critical rearrangement
  • Haematological
  • M-FISH

ASJC Scopus subject areas

  • Hematology

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