Detection of d-aspartate in tau proteins associated with Alzheimer paired helical filaments

Agnes Kenessey, Shu Hui Yen, Wan Kyng Liu, Xiao Ran Yang, David S. Dunlop

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Paired helical filaments (PHF) characteristic of Alzheimer neurofibrillary lesions are known to contain a modified form of microtubule associated protein tau. These proteins, PHF-tau, differ from normal tau in the extent and the site of phosphorylation. To determine whether PHF-tau, tau proteins from normal adult brains (N-tau), tau proteins from Alzheimer brains not associated with PHF (A-tau), and tau proteins from fetal brains (F-tau) differ in racemization, these proteins were compared for their d-aspartate content. The results demonstrated that PHF-tau contain more d-aspartate than N-tau, A-tau and F-tau. The average percentage d-aspartate for these proteins, after a correction for background, are 4.9%, 2.8%, 1.6%, and 1% for PHF-tau, N-tau, A-tau and F-tau, respectively. It remains to be determined if the increase in d-aspartate is a consequence of PHF formation. It is also unknown if the change in d-aspartate content in PHF-tau is associated with phosphorylation, which alters the susceptibility of tau to proteolysis.

Original languageEnglish (US)
Pages (from-to)183-189
Number of pages7
JournalBrain Research
Volume675
Issue number1-2
DOIs
StatePublished - Mar 27 1995
Externally publishedYes

Keywords

  • Alzheimer's disease
  • d-Aspartate
  • Normal tau
  • PHF-tau

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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