TY - JOUR
T1 - Detection of d-aspartate in tau proteins associated with Alzheimer paired helical filaments
AU - Kenessey, Agnes
AU - Yen, Shu Hui
AU - Liu, Wan Kyng
AU - Yang, Xiao Ran
AU - Dunlop, David S.
N1 - Funding Information:
This work was supported by NIH Grants AG01136 and AG04145 (S.-H.Y.). We thank Drs. V.M.-Y. Lee and L.I. Binder for antibodies Tau-46 and Tau-1, respectively. We also thank Dr. D.W. Dickson for his comment on the manuscript.
PY - 1995/3/27
Y1 - 1995/3/27
N2 - Paired helical filaments (PHF) characteristic of Alzheimer neurofibrillary lesions are known to contain a modified form of microtubule associated protein tau. These proteins, PHF-tau, differ from normal tau in the extent and the site of phosphorylation. To determine whether PHF-tau, tau proteins from normal adult brains (N-tau), tau proteins from Alzheimer brains not associated with PHF (A-tau), and tau proteins from fetal brains (F-tau) differ in racemization, these proteins were compared for their d-aspartate content. The results demonstrated that PHF-tau contain more d-aspartate than N-tau, A-tau and F-tau. The average percentage d-aspartate for these proteins, after a correction for background, are 4.9%, 2.8%, 1.6%, and 1% for PHF-tau, N-tau, A-tau and F-tau, respectively. It remains to be determined if the increase in d-aspartate is a consequence of PHF formation. It is also unknown if the change in d-aspartate content in PHF-tau is associated with phosphorylation, which alters the susceptibility of tau to proteolysis.
AB - Paired helical filaments (PHF) characteristic of Alzheimer neurofibrillary lesions are known to contain a modified form of microtubule associated protein tau. These proteins, PHF-tau, differ from normal tau in the extent and the site of phosphorylation. To determine whether PHF-tau, tau proteins from normal adult brains (N-tau), tau proteins from Alzheimer brains not associated with PHF (A-tau), and tau proteins from fetal brains (F-tau) differ in racemization, these proteins were compared for their d-aspartate content. The results demonstrated that PHF-tau contain more d-aspartate than N-tau, A-tau and F-tau. The average percentage d-aspartate for these proteins, after a correction for background, are 4.9%, 2.8%, 1.6%, and 1% for PHF-tau, N-tau, A-tau and F-tau, respectively. It remains to be determined if the increase in d-aspartate is a consequence of PHF formation. It is also unknown if the change in d-aspartate content in PHF-tau is associated with phosphorylation, which alters the susceptibility of tau to proteolysis.
KW - Alzheimer's disease
KW - Normal tau
KW - PHF-tau
KW - d-Aspartate
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U2 - 10.1016/0006-8993(95)00061-T
DO - 10.1016/0006-8993(95)00061-T
M3 - Article
C2 - 7796127
AN - SCOPUS:0028964090
SN - 0006-8993
VL - 675
SP - 183
EP - 189
JO - Brain Research
JF - Brain Research
IS - 1-2
ER -