Abstract
The αβ T cell antigen receptor (TCR) endows T lymphocytes with immune specificity and controls their effector functions. Each person possesses a vast repertoire of TCRs that is generated by the well-studied processes of somatic recombination and thymic selection. While many antibodies specific for TCRβ variable domains are available, antibodies specific for human TCRα are rare. We now report a novel monoclonal antibody, 7F18, which binds to human TCRα constant region, with specificity for a denatured epitope that can be visualized by SDS-PAGE followed by Western blot. Both immature and mature TCR α-chain products can be visualized, making 7F18 potentially applicable to various biochemical assays of multiprotein complex assembly and maturation. This new monoclonal antibody provides a tool that can potentially facilitate the biochemical analysis of comprehensive populations of human αβ TCR complexes that need not be limited to small subsets of the repertoire.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 386-392 |
| Number of pages | 7 |
| Journal | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy |
| Volume | 33 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1 2014 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology