Abstract
The ability of laser-induced fluorescence (LIF) to detect flat dysplasia has not been carefully studied. A multiexcitation wavelength LIF system was used to develop an algorithm to detect chemically-induced dysplasia in the rat urinary bladder. Dysplasia was generated using intravesical doses of N-methyl-N-nitrosourea (MNU). A total of the 108 randomly selected sites, 1 5 untreated controls and 93 MNUtreated, were examined. Of the treated sites, 34% were histologically classified as dysplasia, 1 1% as carcinoma in situ (CIS), 15% as squamous metaplasia, 12% as hyperplasia and 28% as being normal. For fluorescence measurements, 6 excitations wavelengths from 360 nm to 425 nm were used and complete emission spectra recorded. The data were split into neoplastic (dysplastic and CIS sites) and non-neoplastic (normal and hyperplastic sites) groups using 3 different scoring criteria and a stepwise multivariate linear regression analysis was performed on the excitation-emission matrix. In one algorithm, only 3 excitation-emission pairs: (370,400), (370,725), and (415, 475) were needed. Thirtytwo of 40 neoplastic sites and 40 of 45 non-neoplastic sites were correctly classified and an overall accuracy of 8 1% was achieved with this model.
Original language | English (US) |
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Pages (from-to) | 76-79 |
Number of pages | 4 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 2135 |
DOIs | |
State | Published - May 19 1994 |
Event | Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases 1994 - Los Angeles, United States Duration: Jan 23 1994 → Jan 29 1994 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering