Detection of chemically-induced dysplasia in rat urinary bladder with laser-induced fluorescence

Kevin T. Schomacker, Thomas J. Flotte, Thomas F. Deutsch

Research output: Contribution to journalConference articlepeer-review

4 Scopus citations

Abstract

The ability of laser-induced fluorescence (LIF) to detect flat dysplasia has not been carefully studied. A multiexcitation wavelength LIF system was used to develop an algorithm to detect chemically-induced dysplasia in the rat urinary bladder. Dysplasia was generated using intravesical doses of N-methyl-N-nitrosourea (MNU). A total of the 108 randomly selected sites, 1 5 untreated controls and 93 MNUtreated, were examined. Of the treated sites, 34% were histologically classified as dysplasia, 1 1% as carcinoma in situ (CIS), 15% as squamous metaplasia, 12% as hyperplasia and 28% as being normal. For fluorescence measurements, 6 excitations wavelengths from 360 nm to 425 nm were used and complete emission spectra recorded. The data were split into neoplastic (dysplastic and CIS sites) and non-neoplastic (normal and hyperplastic sites) groups using 3 different scoring criteria and a stepwise multivariate linear regression analysis was performed on the excitation-emission matrix. In one algorithm, only 3 excitation-emission pairs: (370,400), (370,725), and (415, 475) were needed. Thirtytwo of 40 neoplastic sites and 40 of 45 non-neoplastic sites were correctly classified and an overall accuracy of 8 1% was achieved with this model.

Original languageEnglish (US)
Pages (from-to)76-79
Number of pages4
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume2135
DOIs
StatePublished - May 19 1994
EventAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases 1994 - Los Angeles, United States
Duration: Jan 23 1994Jan 29 1994

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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