Detection of an element of the SV40 late promoter in vectors used for expression studies in COS cells.

R. Cattaneo, H. Will, G. Darai, E. Pfaff, H. Schaller

Research output: Contribution to journalArticle

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Abstract

Plasmids containing hepatitis B virus (HBV) DNA and a 232-bp SV40 DNA fragment encoding the origin of replication were constructed. When introduced by transfection into COS cells, these plasmids directed the synthesis of hepatitis B surface antigen. S1 mapping of the mRNAs covering the S gene showed that transcriptional initiation was promoted by the interaction of HBV sequences with an SV40 promoter element: transcription started on HBV DNA but had several properties of SV40 late transcription. The detection of a promoter element in an SV40 origin fragment commonly used in the COS system is important for the interpretation of data deriving from expression studies in COS cells.

Original languageEnglish (US)
Pages (from-to)511-514
Number of pages4
JournalEMBO Journal
Volume2
Issue number4
StatePublished - Jan 1 1983

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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