Detection of active hepatitis C virus and hepatitis G virus/GB virus C replication in bone marrow in human subjects

Marek Radkowski, Joanna Kubicka, Elzbieta Kisiel, Janusz Cianciara, Marek Nowicki, Jorge Rakela, Tomasz Laskus

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

We have analyzed the presence of hepatitis C virus (HCV) and hepatitis G virus (HGV) sequences in bone marrow and serum samples from 48 patients of a hematologic outpatient clinic. HCV RNA was detected in 18 (38%) and 15 (31%) and HGV RNA was detected in 6 (13%) and 9 (19%) of serum and bone marrow samples, respectively. In 3 patients, HGV RNA was detectable in bone marrow but not in the serum; 2 of these patients were negative for the presence of specific antibodies. Using a highly strand-specific Tth-based reverse transcriptase-polymerase chain reaction (RT-PCR), the presence of HCV RNA and HGV RNA negative strand was demonstrated in 4 and 5 bone marrow samples, respectively. Our study shows that HCV and HGV can replicate in bone marrow; in the case of HGV, analysis of serum may underestimate the true prevalence of infection. (C) 2000 by The American Society of Hematology.

Original languageEnglish (US)
Pages (from-to)3986-3989
Number of pages4
JournalBlood
Volume95
Issue number12
StatePublished - Jun 15 2000

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Radkowski, M., Kubicka, J., Kisiel, E., Cianciara, J., Nowicki, M., Rakela, J., & Laskus, T. (2000). Detection of active hepatitis C virus and hepatitis G virus/GB virus C replication in bone marrow in human subjects. Blood, 95(12), 3986-3989.