Abstract
A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1-40 and Aβ 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1-40 and Aβ 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 419-423 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 430 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 3 1998 |
| Externally published | Yes |
Keywords
- Alzheimer's disease
- Amyloid β
- HPLC
- Immunoprecipitation
- Mass spectrometry
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
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Cite this
Clarke, N. J., Tomlinson, A. J., Ohyagi, Y., Younkin, S., & Naylor, S. (1998). Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS. FEBS Letters, 430(3), 419-423. https://doi.org/10.1016/S0014-5793(98)00706-6