Abstract
A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1-40 and Aβ 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1-40 and Aβ 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.
Original language | English (US) |
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Pages (from-to) | 419-423 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 430 |
Issue number | 3 |
DOIs | |
State | Published - Jul 3 1998 |
Keywords
- Alzheimer's disease
- Amyloid β
- HPLC
- Immunoprecipitation
- Mass spectrometry
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology