@article{a48858cca0cf48d4959607ac083f444c,
title = "Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS",
abstract = "A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1-40 and Aβ 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1-40 and Aβ 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.",
keywords = "Alzheimer's disease, Amyloid β, HPLC, Immunoprecipitation, Mass spectrometry",
author = "Clarke, {Nigel J.} and Tomlinson, {Andy J.} and Yasumasa Ohyagi and Steven Younkin and Stephen Naylor",
note = "Funding Information: lished results; and J. Isaacson for valuable discussions and comments on the manuscript. Special thanks go to R. RatliIE for providing advice, the hospitality of his laboratory and help of all sorts during our preparation of polynucleotide terminal transferase. We would like to thank R. Firtel for gifts of the phage lambda exonidease and endonrielease R1, R. Roherts and T. Maniatis for gifts of endonuclease Hae I11 and lambda exonuclease, and B. Wallace for his help with the photography. The reverse transcriptase iiscd in this work was provided by the Office of Program Resources and Logistics, Viral Cancer Program Viral Oncology, Division of Cancer Cause and Prevention, and the National Cancer Institute. Funding Information: The research reported was supported in part by USPHS grants GM 18586 and CA 15940. W. S. is a recipient of Public Health Service Career Developnient Award GM 70045. J. B. was snpported in part by USPHS Molecular Biology Training Grant GM 1531, R. H. and €1. H. by training grant CA 09056, and P. Z. by Training Grant GM 7104. G. P. has been supported in part by a Helen Hay Whitney Postdoctoral Fellowship.",
year = "1998",
month = jul,
day = "3",
doi = "10.1016/S0014-5793(98)00706-6",
language = "English (US)",
volume = "430",
pages = "419--423",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",
}