Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS

Nigel J. Clarke; Andy J. Tomlinson; Yasumasa Ohyagi; Steven Younkin; Stephen Naylor

doi:10.1016/S0014-5793(98)00706-6

Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS

Nigel J. Clarke, Andy J. Tomlinson, Yasumasa Ohyagi, Steven Younkin, Stephen Naylor

Neuroscience

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1-40 and Aβ 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1-40 and Aβ 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.

Original language	English (US)
Pages (from-to)	419-423
Number of pages	5
Journal	FEBS Letters
Volume	430
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00706-6
State	Published - Jul 3 1998

Keywords

Alzheimer's disease
Amyloid β
HPLC
Immunoprecipitation
Mass spectrometry

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS",

abstract = "A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1-40 and Aβ 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1-40 and Aβ 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.",

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AU - Clarke, Nigel J.

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AU - Ohyagi, Yasumasa

AU - Younkin, Steven

AU - Naylor, Stephen

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KW - Amyloid β

KW - HPLC

KW - Immunoprecipitation

KW - Mass spectrometry

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