Detection and quantitation of cellularly derived amyloid β peptides by immunoprecipitation-HPLC-MS

Nigel J. Clarke, Andy J. Tomlinson, Yasumasa Ohyagi, Steven Younkin, Stephen Naylor

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

A quantitative method for detection of amyloid β peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of Aβ 1-40 and Aβ 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for Aβ 1-40 and Aβ 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other Aβ peptide species that may also play a role in the onset of AD in humans.

Original languageEnglish (US)
Pages (from-to)419-423
Number of pages5
JournalFEBS Letters
Volume430
Issue number3
DOIs
StatePublished - Jul 3 1998
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Amyloid β
  • HPLC
  • Immunoprecipitation
  • Mass spectrometry

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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