Detection and monitoring of neurotransmitters - A spectroscopic analysis

Felicia S. Manciu, Kendall H Lee, William G. Durrer, Kevin E. Bennet

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: We demonstrate that confocal Raman mapping spectroscopy provides rapid, detailed, and accurate neurotransmitter analysis, enabling millisecond time resolution monitoring of biochemical dynamics. As a prototypical demonstration of the power of the method, we present real-time in vitro serotonin, adenosine, and dopamine detection, and dopamine diffusion in an inhomogeneous organic gel, which was used as a substitute for neurologic tissue. Materials and Methods: Dopamine, adenosine, and serotonin were used to prepare neurotransmitter solutions in distilled water. The solutions were applied to the surfaces of glass slides, where they interdiffused. Raman mapping was achieved by detecting nonoverlapping spectral signatures characteristic of the neurotransmitters with an alpha 300 WITec confocal Raman system, using 532 nm neodymium-doped yttrium aluminum garnet laser excitation. Every local Raman spectrum was recorded in milliseconds and complete Raman mapping in a few seconds. Results: Without damage, dyeing, or preferential sample preparation, confocal Raman mapping provided positive detection of each neurotransmitter, allowing association of the high-resolution spectra with specific microscale image regions. Such information is particularly important for complex, heterogeneous samples, where changes in composition can influence neurotransmission processes. We also report an estimated dopamine diffusion coefficient two orders of magnitude smaller than that calculated by the flow-injection method. Conclusions: Accurate nondestructive characterization for real-time detection of neurotransmitters in inhomogeneous environments without the requirement of sample labeling is a key issue in neuroscience. Our work demonstrates the capabilities of Raman spectroscopy in biological applications, possibly providing a new tool for elucidating the mechanism and kinetics of deep brain stimulation.

Original languageEnglish (US)
Pages (from-to)192-197
Number of pages6
JournalNeuromodulation
Volume16
Issue number3
DOIs
StatePublished - May 2013

Fingerprint

Neurotransmitter Agents
Dopamine
Raman Spectrum Analysis
Adenosine
Serotonin
Deep Brain Stimulation
Solid-State Lasers
Neurosciences
Synaptic Transmission
Nervous System
Glass
Gels
Injections
Water

Keywords

  • Basic science
  • brain
  • neurotransmitters
  • Raman spectroscopy

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Neurology
  • Clinical Neurology

Cite this

Detection and monitoring of neurotransmitters - A spectroscopic analysis. / Manciu, Felicia S.; Lee, Kendall H; Durrer, William G.; Bennet, Kevin E.

In: Neuromodulation, Vol. 16, No. 3, 05.2013, p. 192-197.

Research output: Contribution to journalArticle

Manciu, Felicia S. ; Lee, Kendall H ; Durrer, William G. ; Bennet, Kevin E. / Detection and monitoring of neurotransmitters - A spectroscopic analysis. In: Neuromodulation. 2013 ; Vol. 16, No. 3. pp. 192-197.
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