Detection and enumeration of circulating tumor cells based on their invasive property

Haizhen Wang, Yannis Hara, Xingtong Liu, James M. Reuben, Yongzhuang Xie, Huaxi Xu, Guojun Bu, Yihua Pei, Vineet Gupta, Xiangwei Wu

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Circulating tumor cells (CTCs) are in limited numbers and heterogeneous, making their detection, isolation, and enumeration a major challenge. To overcome these difficulties, we developed a novel method to detect and enumerate CTCs with invasive property. Our assay consists of three simple steps: enrichment, Matrigel invasion assay, and immunostaining. We have validated this method using mouse xenograft tumor models and confirmed its utility in human cancer patients. Our method does not require special equipment and antigen expression for CTC selection, is less likely to be affected by the heterogeneity of the CTCs, and could be applicable to virtually all cancers. Most important, our method enumerates invasive CTCs, which may allow more accurate correlations with clinical outcome and treatment response compared with other CTC detection methods.

Original languageEnglish (US)
Pages (from-to)27304-27311
Number of pages8
JournalOncotarget
Volume6
Issue number29
DOIs
StatePublished - 2015

Keywords

  • Cell invasion assay
  • Circulating tumor cells
  • Functional property
  • Metastasis

ASJC Scopus subject areas

  • Oncology

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  • Cite this

    Wang, H., Hara, Y., Liu, X., Reuben, J. M., Xie, Y., Xu, H., Bu, G., Pei, Y., Gupta, V., & Wu, X. (2015). Detection and enumeration of circulating tumor cells based on their invasive property. Oncotarget, 6(29), 27304-27311. https://doi.org/10.18632/oncotarget.4454