Abstract
In cultured cells, an increase in cellular levels of reactive oxygen species (ROS) can be detected using multiple techniques including colorimetric assays, immunoblotting, and immunofluorescence. These methods can also be applied for ROS measurement in tissue samples, but often require tissue homogenization, and therefore do not distinguish between the different cell types within a tissue. Here, we describe a detailed protocol for determination of altered oxidative stress levels in different cell types in tissues, by detecting ROS-caused alteration of macromolecules using immunohistochemistry (IHC). This method is demonstrated by using 4HNE as a marker for lipid peroxidation in mouse pancreas tissue that contains precancerous lesions high in cellular oxidative stress.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 97-104 |
| Number of pages | 8 |
| Journal | Methods in Molecular Biology |
| Volume | 1292 |
| DOIs | |
| State | Published - 2015 |
Fingerprint
Keywords
- 4HNE
- Immunohistochemistry
- Lipid peroxidation
- Oxidative stress
- Reactive oxygen species
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Detecting reactive oxygen species by immunohistochemistry. / Liou, Geou Yarh; Storz, Peter.
In: Methods in Molecular Biology, Vol. 1292, 2015, p. 97-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detecting reactive oxygen species by immunohistochemistry
AU - Liou, Geou Yarh
AU - Storz, Peter
PY - 2015
Y1 - 2015
N2 - In cultured cells, an increase in cellular levels of reactive oxygen species (ROS) can be detected using multiple techniques including colorimetric assays, immunoblotting, and immunofluorescence. These methods can also be applied for ROS measurement in tissue samples, but often require tissue homogenization, and therefore do not distinguish between the different cell types within a tissue. Here, we describe a detailed protocol for determination of altered oxidative stress levels in different cell types in tissues, by detecting ROS-caused alteration of macromolecules using immunohistochemistry (IHC). This method is demonstrated by using 4HNE as a marker for lipid peroxidation in mouse pancreas tissue that contains precancerous lesions high in cellular oxidative stress.
AB - In cultured cells, an increase in cellular levels of reactive oxygen species (ROS) can be detected using multiple techniques including colorimetric assays, immunoblotting, and immunofluorescence. These methods can also be applied for ROS measurement in tissue samples, but often require tissue homogenization, and therefore do not distinguish between the different cell types within a tissue. Here, we describe a detailed protocol for determination of altered oxidative stress levels in different cell types in tissues, by detecting ROS-caused alteration of macromolecules using immunohistochemistry (IHC). This method is demonstrated by using 4HNE as a marker for lipid peroxidation in mouse pancreas tissue that contains precancerous lesions high in cellular oxidative stress.
KW - 4HNE
KW - Immunohistochemistry
KW - Lipid peroxidation
KW - Oxidative stress
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84961353113&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961353113&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2522-3_7
DO - 10.1007/978-1-4939-2522-3_7
M3 - Article
C2 - 25804750
AN - SCOPUS:84961353113
VL - 1292
SP - 97
EP - 104
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -