Detachment of adherent human monocytes from glass surfaces induced by antibody-coated and uncoated erythrocytes

N. E. Kay, S. K. Ackerman, S. D. Douglas

Research output: Contribution to journalArticlepeer-review

Abstract

Monocyte adherence to glass surface is an essential step in most monocyte purification methods. In order to evaluate the stability of monocyte attachment to glass surfaces we utilized a spectrofluorometric DNA assay to determine numbers of human mononuclear cells which were adherent to glass. The addition of erythrocytes or of erythrocytes coated with IgM plus C3 to the adherent-cell population resulted in significant and comparable detachment of monocytes; greater detachment occurred with IgG-coated erythrocytes. Nonimmune phagocytosis was also associated with detachment, while Fc receptor occupancy by IgG was not. These results suggest that (i) monocyte-glass surface attachment may be modified by cell-cell interactions; and (ii) that the detachment assay may detect interactions between monocytes and other cells (e.g., uncoated erythrocytes) not detected by assays of phagocytosis or rosette formation.

Original languageEnglish (US)
Pages (from-to)269-276
Number of pages8
JournalClinical Immunology and Immunopathology
Volume13
Issue number3
DOIs
StatePublished - Jul 1979

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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