Monocyte adherence to glass surface is an essential step in most monocyte purification methods. In order to evaluate the stability of monocyte attachment to glass surfaces we utilized a spectrofluorometric DNA assay to determine numbers of human mononuclear cells which were adherent to glass. The addition of erythrocytes or of erythrocytes coated with IgM plus C3 to the adherent-cell population resulted in significant and comparable detachment of monocytes; greater detachment occurred with IgG-coated erythrocytes. Nonimmune phagocytosis was also associated with detachment, while Fc receptor occupancy by IgG was not. These results suggest that (i) monocyte-glass surface attachment may be modified by cell-cell interactions; and (ii) that the detachment assay may detect interactions between monocytes and other cells (e.g., uncoated erythrocytes) not detected by assays of phagocytosis or rosette formation.
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine