Desired and side effects of the supplementation with l-glutamine and l-glutathione in enteric glia of diabetic rats

Cynthia Priscilla do Nascimento Bonato Panizzon, Jacqueline Nelisis Zanoni, Catchia Hermes-Uliana, Aline Rosa Trevizan, Camila Caviquioli Sehaber, Renata Virginia Fernandes Pereira, David R Linden, Marcílio Hubner de Miranda Neto

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background/Aims: Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2% . l-glutamine and 1% . l-glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods: Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with . l-glutamine (NG), Normoglycemic supplemented with . l-glutathione (NGO), Diabetic (D), Diabetic supplemented with . l-glutamine (DG) and Diabetic supplemented with . l-glutathione (DGO). After 120. days, the ileum was processed for immunohistochemistry of HuC/D and S100β. Quantitative and morphometric analysis have been performed. Results: Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. . l-Glutathione prevented the neuronal death in diabetic rats. . l-Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with . l-glutamine and . l-glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with . l-glutamine and . l-glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion: It is concluded that the usage of . l-glutamine and . l-glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals.

Original languageEnglish (US)
JournalActa Histochemica
DOIs
StateAccepted/In press - Apr 18 2016

Keywords

  • Antioxidants
  • Diabetes mellitus
  • Enteric nervous system
  • Glia cells

ASJC Scopus subject areas

  • Cell Biology
  • Histology

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    Panizzon, C. P. D. N. B., Zanoni, J. N., Hermes-Uliana, C., Trevizan, A. R., Sehaber, C. C., Pereira, R. V. F., Linden, D. R., & Neto, M. H. D. M. (Accepted/In press). Desired and side effects of the supplementation with l-glutamine and l-glutathione in enteric glia of diabetic rats. Acta Histochemica. https://doi.org/10.1016/j.acthis.2016.07.008