TY - JOUR
T1 - Desired and side effects of the supplementation with L-glutamine and L-glutathione in enteric glia of diabetic rats
AU - Panizzon, Cynthia Priscilla do Nascimento Bonato
AU - Zanoni, Jacqueline Nelisis
AU - Hermes-Uliana, Catchia
AU - Trevizan, Aline Rosa
AU - Sehaber, Camila Caviquioli
AU - Pereira, Renata Virginia Fernandes
AU - Linden, David Robert
AU - Neto, Marcílio Hubner de Miranda
N1 - Funding Information:
This work was supported by Fundação Araucaria-PR and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). DRL was supported by NIH grant DK106011.
Publisher Copyright:
© 2016 Elsevier GmbH
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Background/Aims Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2% L-glutamine and 1% L-glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with L-glutamine (NG), Normoglycemic supplemented with L-glutathione (NGO), Diabetic (D), Diabetic supplemented with L-glutamine (DG) and Diabetic supplemented with L-glutathione (DGO). After 120 days, the ileum was processed for immunohistochemistry of HuC/D and S100β. Quantitative and morphometric analysis have been performed. Results Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. L-Glutathione prevented the neuronal death in diabetic rats. L-Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with L-glutamine and L-glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with L-glutamine and L-glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion It is concluded that the usage of L-glutamine and L-glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals.
AB - Background/Aims Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2% L-glutamine and 1% L-glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with L-glutamine (NG), Normoglycemic supplemented with L-glutathione (NGO), Diabetic (D), Diabetic supplemented with L-glutamine (DG) and Diabetic supplemented with L-glutathione (DGO). After 120 days, the ileum was processed for immunohistochemistry of HuC/D and S100β. Quantitative and morphometric analysis have been performed. Results Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. L-Glutathione prevented the neuronal death in diabetic rats. L-Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with L-glutamine and L-glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with L-glutamine and L-glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion It is concluded that the usage of L-glutamine and L-glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals.
KW - Antioxidants
KW - Diabetes mellitus
KW - Enteric nervous system
KW - Glia cells
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U2 - 10.1016/j.acthis.2016.07.008
DO - 10.1016/j.acthis.2016.07.008
M3 - Article
C2 - 27470531
AN - SCOPUS:84979679222
SN - 0065-1281
VL - 118
SP - 625
EP - 631
JO - Acta Histochemica
JF - Acta Histochemica
IS - 6
ER -