Desired and side effects of the supplementation with l-glutamine and l-glutathione in enteric glia of diabetic rats

Cynthia Priscilla do Nascimento Bonato Panizzon, Jacqueline Nelisis Zanoni, Catchia Hermes-Uliana, Aline Rosa Trevizan, Camila Caviquioli Sehaber, Renata Virginia Fernandes Pereira, David R Linden, Marcílio Hubner de Miranda Neto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background/Aims: Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2% . l-glutamine and 1% . l-glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods: Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with . l-glutamine (NG), Normoglycemic supplemented with . l-glutathione (NGO), Diabetic (D), Diabetic supplemented with . l-glutamine (DG) and Diabetic supplemented with . l-glutathione (DGO). After 120. days, the ileum was processed for immunohistochemistry of HuC/D and S100β. Quantitative and morphometric analysis have been performed. Results: Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. . l-Glutathione prevented the neuronal death in diabetic rats. . l-Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with . l-glutamine and . l-glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with . l-glutamine and . l-glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion: It is concluded that the usage of . l-glutamine and . l-glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals.

Original languageEnglish (US)
JournalActa Histochemica
DOIs
StateAccepted/In press - Apr 18 2016

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Glutamine
Neuroglia
Glutathione
Ileum
Intestinal Pseudo-Obstruction
Neurons
Digestive System
Constipation
Nausea
Vomiting
Wistar Rats
Diarrhea
Diabetes Mellitus
Immunohistochemistry

Keywords

  • Antioxidants
  • Diabetes mellitus
  • Enteric nervous system
  • Glia cells

ASJC Scopus subject areas

  • Cell Biology
  • Histology

Cite this

Panizzon, C. P. D. N. B., Zanoni, J. N., Hermes-Uliana, C., Trevizan, A. R., Sehaber, C. C., Pereira, R. V. F., ... Neto, M. H. D. M. (Accepted/In press). Desired and side effects of the supplementation with l-glutamine and l-glutathione in enteric glia of diabetic rats. Acta Histochemica. https://doi.org/10.1016/j.acthis.2016.07.008

Desired and side effects of the supplementation with l-glutamine and l-glutathione in enteric glia of diabetic rats. / Panizzon, Cynthia Priscilla do Nascimento Bonato; Zanoni, Jacqueline Nelisis; Hermes-Uliana, Catchia; Trevizan, Aline Rosa; Sehaber, Camila Caviquioli; Pereira, Renata Virginia Fernandes; Linden, David R; Neto, Marcílio Hubner de Miranda.

In: Acta Histochemica, 18.04.2016.

Research output: Contribution to journalArticle

Panizzon, Cynthia Priscilla do Nascimento Bonato ; Zanoni, Jacqueline Nelisis ; Hermes-Uliana, Catchia ; Trevizan, Aline Rosa ; Sehaber, Camila Caviquioli ; Pereira, Renata Virginia Fernandes ; Linden, David R ; Neto, Marcílio Hubner de Miranda. / Desired and side effects of the supplementation with l-glutamine and l-glutathione in enteric glia of diabetic rats. In: Acta Histochemica. 2016.
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abstract = "Background/Aims: Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2{\%} . l-glutamine and 1{\%} . l-glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods: Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with . l-glutamine (NG), Normoglycemic supplemented with . l-glutathione (NGO), Diabetic (D), Diabetic supplemented with . l-glutamine (DG) and Diabetic supplemented with . l-glutathione (DGO). After 120. days, the ileum was processed for immunohistochemistry of HuC/D and S100β. Quantitative and morphometric analysis have been performed. Results: Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. . l-Glutathione prevented the neuronal death in diabetic rats. . l-Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with . l-glutamine and . l-glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with . l-glutamine and . l-glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion: It is concluded that the usage of . l-glutamine and . l-glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals.",
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T1 - Desired and side effects of the supplementation with l-glutamine and l-glutathione in enteric glia of diabetic rats

AU - Panizzon, Cynthia Priscilla do Nascimento Bonato

AU - Zanoni, Jacqueline Nelisis

AU - Hermes-Uliana, Catchia

AU - Trevizan, Aline Rosa

AU - Sehaber, Camila Caviquioli

AU - Pereira, Renata Virginia Fernandes

AU - Linden, David R

AU - Neto, Marcílio Hubner de Miranda

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N2 - Background/Aims: Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2% . l-glutamine and 1% . l-glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods: Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with . l-glutamine (NG), Normoglycemic supplemented with . l-glutathione (NGO), Diabetic (D), Diabetic supplemented with . l-glutamine (DG) and Diabetic supplemented with . l-glutathione (DGO). After 120. days, the ileum was processed for immunohistochemistry of HuC/D and S100β. Quantitative and morphometric analysis have been performed. Results: Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. . l-Glutathione prevented the neuronal death in diabetic rats. . l-Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with . l-glutamine and . l-glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with . l-glutamine and . l-glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion: It is concluded that the usage of . l-glutamine and . l-glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals.

AB - Background/Aims: Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2% . l-glutamine and 1% . l-glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods: Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with . l-glutamine (NG), Normoglycemic supplemented with . l-glutathione (NGO), Diabetic (D), Diabetic supplemented with . l-glutamine (DG) and Diabetic supplemented with . l-glutathione (DGO). After 120. days, the ileum was processed for immunohistochemistry of HuC/D and S100β. Quantitative and morphometric analysis have been performed. Results: Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. . l-Glutathione prevented the neuronal death in diabetic rats. . l-Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with . l-glutamine and . l-glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with . l-glutamine and . l-glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion: It is concluded that the usage of . l-glutamine and . l-glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals.

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KW - Glia cells

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