Abstract
In this paper, we demonstrate the use of individually addressable microelectrodes for cell sorting and cell micropatterning applications. Microelectrodes were modified with cell adhesive or non-adhesive molecules and then electrically stimulated to selectively adsorb or desorb proteins and/or mammalian cells. The switching of the surface properties was achieved by the electrochemical desorption of protein-functionalized thiols and poly(ethylene glycol) PEG silane from gold and indium tin oxide (ITO) electrodes respectively. The thiol surfaces were modified with anti-CD4 antibodies and used to capture T-cells. Upon electrical activation of the microelectrodes, both the antibodies and the T-cells were removed from the specific locations on the substrate. In addition, ITO electrodes were modified with cellresistive PEG silane which was later electrochemically desorbed to make the surface adhesive to proteins or cells. This technique was employed to pattern two different cell types on the same substrate.
| Original language | English (US) |
|---|---|
| Title of host publication | ECS Transactions - Electrochemical Nano/Biosensors |
| Pages | 17-25 |
| Number of pages | 9 |
| Volume | 13 |
| Edition | 22 |
| DOIs | |
| State | Published - Dec 1 2008 |
| Externally published | Yes |
| Event | Electrochemical Nano/Biosensors - 213th Meeting of the Electrochemical Society - Phoenix, AZ, United States Duration: May 18 2008 → May 23 2009 |
Other
| Other | Electrochemical Nano/Biosensors - 213th Meeting of the Electrochemical Society |
|---|---|
| Country | United States |
| City | Phoenix, AZ |
| Period | 5/18/08 → 5/23/09 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Designing electroactive biointerface for spatiotemporal control of cell attachment and release. / Shah, Sunny; Zhu, He; Yan, Jun; Verkhoturov, Stanislav; Revzin, Alexander.
ECS Transactions - Electrochemical Nano/Biosensors. Vol. 13 22. ed. 2008. p. 17-25.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Designing electroactive biointerface for spatiotemporal control of cell attachment and release
AU - Shah, Sunny
AU - Zhu, He
AU - Yan, Jun
AU - Verkhoturov, Stanislav
AU - Revzin, Alexander
PY - 2008/12/1
Y1 - 2008/12/1
N2 - In this paper, we demonstrate the use of individually addressable microelectrodes for cell sorting and cell micropatterning applications. Microelectrodes were modified with cell adhesive or non-adhesive molecules and then electrically stimulated to selectively adsorb or desorb proteins and/or mammalian cells. The switching of the surface properties was achieved by the electrochemical desorption of protein-functionalized thiols and poly(ethylene glycol) PEG silane from gold and indium tin oxide (ITO) electrodes respectively. The thiol surfaces were modified with anti-CD4 antibodies and used to capture T-cells. Upon electrical activation of the microelectrodes, both the antibodies and the T-cells were removed from the specific locations on the substrate. In addition, ITO electrodes were modified with cellresistive PEG silane which was later electrochemically desorbed to make the surface adhesive to proteins or cells. This technique was employed to pattern two different cell types on the same substrate.
AB - In this paper, we demonstrate the use of individually addressable microelectrodes for cell sorting and cell micropatterning applications. Microelectrodes were modified with cell adhesive or non-adhesive molecules and then electrically stimulated to selectively adsorb or desorb proteins and/or mammalian cells. The switching of the surface properties was achieved by the electrochemical desorption of protein-functionalized thiols and poly(ethylene glycol) PEG silane from gold and indium tin oxide (ITO) electrodes respectively. The thiol surfaces were modified with anti-CD4 antibodies and used to capture T-cells. Upon electrical activation of the microelectrodes, both the antibodies and the T-cells were removed from the specific locations on the substrate. In addition, ITO electrodes were modified with cellresistive PEG silane which was later electrochemically desorbed to make the surface adhesive to proteins or cells. This technique was employed to pattern two different cell types on the same substrate.
UR - http://www.scopus.com/inward/record.url?scp=65649090158&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65649090158&partnerID=8YFLogxK
U2 - 10.1149/1.3005399
DO - 10.1149/1.3005399
M3 - Conference contribution
AN - SCOPUS:65649090158
SN - 9781605606606
VL - 13
SP - 17
EP - 25
BT - ECS Transactions - Electrochemical Nano/Biosensors
ER -