Designing electroactive biointerface for spatiotemporal control of cell attachment and release

Sunny Shah, He Zhu, Jun Yan, Stanislav Verkhoturov, Alexander Revzin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we demonstrate the use of individually addressable microelectrodes for cell sorting and cell micropatterning applications. Microelectrodes were modified with cell adhesive or non-adhesive molecules and then electrically stimulated to selectively adsorb or desorb proteins and/or mammalian cells. The switching of the surface properties was achieved by the electrochemical desorption of protein-functionalized thiols and poly(ethylene glycol) PEG silane from gold and indium tin oxide (ITO) electrodes respectively. The thiol surfaces were modified with anti-CD4 antibodies and used to capture T-cells. Upon electrical activation of the microelectrodes, both the antibodies and the T-cells were removed from the specific locations on the substrate. In addition, ITO electrodes were modified with cellresistive PEG silane which was later electrochemically desorbed to make the surface adhesive to proteins or cells. This technique was employed to pattern two different cell types on the same substrate.

Original languageEnglish (US)
Title of host publicationECS Transactions - Electrochemical Nano/Biosensors
PublisherElectrochemical Society Inc.
Pages17-25
Number of pages9
Edition22
ISBN (Electronic)9781566776806
DOIs
StatePublished - 2008
EventElectrochemical Nano/Biosensors - 213th Meeting of the Electrochemical Society - Phoenix, AZ, United States
Duration: May 18 2008May 23 2009

Publication series

NameECS Transactions
Number22
Volume13
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Conference

ConferenceElectrochemical Nano/Biosensors - 213th Meeting of the Electrochemical Society
Country/TerritoryUnited States
CityPhoenix, AZ
Period5/18/085/23/09

ASJC Scopus subject areas

  • General Engineering

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