Design and characterization of a homodimeric antiparallel coiled coil

Daniel G. Gurnon, Jennifer A. Whitaker, Martha G. Oakley

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

We report the first successful design of a self-associating antiparallel coiled coil, APH. The simultaneous application of Coulombic and hydrophobic components results in a decided preference for the antiparallel alignment as judged by HPLC, sedimentation equilibrium, and chemical denaturation data. The designed peptide is of comparable stability to naturally occurring leucine zipper peptides and can be expressed in bacteria. These properties of APH suggest potential in vivo protein fusion and biomaterials applications.

Original languageEnglish (US)
Pages (from-to)7518-7519
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number25
DOIs
StatePublished - Jun 25 2003

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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