Description and validation of isometric tetanic muscle force test in rabbits

Guilherme Giusti, Thomas Kremer, Wouter F. Willems, Patricia F. Friedrich, Allen Thorp Bishop, Alexander Yong-Shik Shin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Isometric tetanic muscle force has been described in a rat model to evaluate motor recovery in a segmental sciatic nerve defect reconstructions. However, to test longer nerve defects, an alternative and larger animal model is necessary. The purpose of this study is to describe and validate a technique for isometric force measurement of the tibialis anterior (TA) muscle in New Zealand rabbits. Muscle preload and electrical stimulation parameters were optimized to obtain the highest tetanic contraction bilaterally in 10 animals. Electrophysiology, muscle weight, peroneal nerve length, and histomorphometry were also analyzed. Only the peroneal nerve length and the ratio of highest muscle force/muscle weight demonstrated the equivalence between the sides. A small variability of TA muscle force and TA muscle weight was observed between the sides suggesting dominance. Optimization of electrical stimulation and preload as well as the use of correct anesthesia were fundamental to acquire the highest muscle force.

Original languageEnglish (US)
Pages (from-to)35-42
Number of pages8
JournalMicrosurgery
Volume32
Issue number1
DOIs
StatePublished - Jan 2012

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Rabbits
Muscles
Peroneal Nerve
Weights and Measures
Electric Stimulation
Electrophysiology
Sciatic Nerve
Anesthesia
Animal Models

ASJC Scopus subject areas

  • Surgery

Cite this

Description and validation of isometric tetanic muscle force test in rabbits. / Giusti, Guilherme; Kremer, Thomas; Willems, Wouter F.; Friedrich, Patricia F.; Bishop, Allen Thorp; Shin, Alexander Yong-Shik.

In: Microsurgery, Vol. 32, No. 1, 01.2012, p. 35-42.

Research output: Contribution to journalArticle

Giusti, Guilherme ; Kremer, Thomas ; Willems, Wouter F. ; Friedrich, Patricia F. ; Bishop, Allen Thorp ; Shin, Alexander Yong-Shik. / Description and validation of isometric tetanic muscle force test in rabbits. In: Microsurgery. 2012 ; Vol. 32, No. 1. pp. 35-42.
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