Dependence of plasmin-mediated degradation of platelet adhesive receptors on temperature and Ca2+

Kenneth J. Winters, Paul R. Eisenberg, Allan S Jaffe, Samuel A. Santoro

Research output: Contribution to journalArticle

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Abstract

The effects of activation of plasminogen by streptokinase and tissue-type-plasminogen activator on platelet activation and the membrane glycoproteins (GPs) that mediate platelet adhesion and aggregation are not yet fully defined. To clarify effects on platelets during activation of plasminogen in vitro, we used monoclonal antibodies (MoAbs), flow cytometry. and platelets surface-labeled with 125I to characterize changes in receptors for fibrinogen (GPIIb-IIIa), von Willebrand factor (GPIb), and collagen (GPIa-IIa). Activation of plasminogen in plasma with pharmacologic concentrations of plasminogen activators did not degrade GPIIb-IIIa or GPIb, and caused only a modest decrease in GPIa. In washed platelets GPIIb-IIIa was extensively degraded by plasmin at 37°C in the absence of exogenous Ca2+, conditions that destabilize the Hb-IIIa complex. Degradation of GPIb in washed platelets displayed a similar although less-marked dependence on temperature and the absence of Ca2+ . The binding of activation-specific MoAbs did not increase during activation of plasminogen in plasma. We conclude that during pharmacologic fibrinolysis, reported inhibition of platelet function in plasma is not due to degradation of platelet-adhesive receptors. In addition, platelet activation observed during thrombolytic therapy does not appear to be a direct consequence of plasminogen activation.

Original languageEnglish (US)
Pages (from-to)1546-1557
Number of pages12
JournalBlood
Volume76
Issue number8
StatePublished - Oct 15 1990
Externally publishedYes

Fingerprint

Plasminogen
Fibrinolysin
Platelets
Adhesives
Blood Platelets
Chemical activation
Integrin alpha2
Platelet Activation
Degradation
Temperature
Monoclonal Antibodies
Platelet Membrane Glycoproteins
Plasmas
Platelet Glycoprotein GPIIb-IIIa Complex
Streptokinase
Plasminogen Activators
Thrombolytic Therapy
von Willebrand Factor
Fibrinolysis
Tissue Plasminogen Activator

ASJC Scopus subject areas

  • Hematology

Cite this

Winters, K. J., Eisenberg, P. R., Jaffe, A. S., & Santoro, S. A. (1990). Dependence of plasmin-mediated degradation of platelet adhesive receptors on temperature and Ca2+ Blood, 76(8), 1546-1557.

Dependence of plasmin-mediated degradation of platelet adhesive receptors on temperature and Ca2+ . / Winters, Kenneth J.; Eisenberg, Paul R.; Jaffe, Allan S; Santoro, Samuel A.

In: Blood, Vol. 76, No. 8, 15.10.1990, p. 1546-1557.

Research output: Contribution to journalArticle

Winters, KJ, Eisenberg, PR, Jaffe, AS & Santoro, SA 1990, 'Dependence of plasmin-mediated degradation of platelet adhesive receptors on temperature and Ca2+ ', Blood, vol. 76, no. 8, pp. 1546-1557.
Winters, Kenneth J. ; Eisenberg, Paul R. ; Jaffe, Allan S ; Santoro, Samuel A. / Dependence of plasmin-mediated degradation of platelet adhesive receptors on temperature and Ca2+ In: Blood. 1990 ; Vol. 76, No. 8. pp. 1546-1557.
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