TY - JOUR
T1 - Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength
T2 - Implications in receptor solubilization
AU - Chattopadhyay, Amitabha
AU - Harikumar, K. G.
N1 - Funding Information:
AcknowledgementTsh: is work wass upportebdy a grant( BT/R and D/9/5/93f)r om the Departmenotf BiotechnologGy,o vernmenotf India, to A.C. Someo f the preliminarsyo lubilizatioenx periments wered oneb y oneo f us (A.C.) as a Wood-WhelaFne llow( offered by theI nternationaUln iono f Biochemistaryn dM oleculaBr iology) at theU niversityo f CaliforniaS, antaC ruz,C A. K.G.H. thankst he Departmenotf BiotechnologGyo, vernmeonft India,f or thea wardo f a postdoctorfael llowshipW. e thankD rs.S. HarinarayanRaa o and C.K. Thota for helpw ith the tissuec ollectiona, nd Dr. Howard Wang,R ebekahD eVinneya nd SatindeRr awatf or helpfuld iscus-sions.
PY - 1996/8/5
Y1 - 1996/8/5
N2 - The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1- propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT(1A)) from bovine brain hippocampal crude (native) membrane by CHAPS at pre- micellar concentration under high salt conditions.
AB - The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1- propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT(1A)) from bovine brain hippocampal crude (native) membrane by CHAPS at pre- micellar concentration under high salt conditions.
KW - 3-[(3-Cholamidopropyl)- dimethylammonio]-1-propane-sulfonate
KW - 5-HT(1A) receptor
KW - CHAPS
KW - Critical micelle concentration
KW - Ionic strength
KW - Solubilization
KW - Zwitterionic detergent
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U2 - 10.1016/0014-5793(96)00733-8
DO - 10.1016/0014-5793(96)00733-8
M3 - Article
C2 - 8706916
AN - SCOPUS:0030570797
SN - 0014-5793
VL - 391
SP - 199
EP - 202
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -