Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: Implications in receptor solubilization

Amitabha Chattopadhyay, K. G. Harikumar

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1- propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT(1A)) from bovine brain hippocampal crude (native) membrane by CHAPS at pre- micellar concentration under high salt conditions.

Original languageEnglish (US)
Pages (from-to)199-202
Number of pages4
JournalFEBS Letters
Volume391
Issue number1-2
DOIs
StatePublished - Aug 5 1996
Externally publishedYes

Fingerprint

Critical micelle concentration
Micelles
Ionic strength
Detergents
Osmolar Concentration
Salts
Membranes
Receptor, Serotonin, 5-HT1A
Brain
Serotonin
Membrane Proteins
propylsulfonic acid

Keywords

  • 3-[(3-Cholamidopropyl)- dimethylammonio]-1-propane-sulfonate
  • 5-HT(1A) receptor
  • CHAPS
  • Critical micelle concentration
  • Ionic strength
  • Solubilization
  • Zwitterionic detergent

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength : Implications in receptor solubilization. / Chattopadhyay, Amitabha; Harikumar, K. G.

In: FEBS Letters, Vol. 391, No. 1-2, 05.08.1996, p. 199-202.

Research output: Contribution to journalArticle

@article{691cb718a6874458bb87aade69ea109a,
title = "Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: Implications in receptor solubilization",
abstract = "The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1- propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT(1A)) from bovine brain hippocampal crude (native) membrane by CHAPS at pre- micellar concentration under high salt conditions.",
keywords = "3-[(3-Cholamidopropyl)- dimethylammonio]-1-propane-sulfonate, 5-HT(1A) receptor, CHAPS, Critical micelle concentration, Ionic strength, Solubilization, Zwitterionic detergent",
author = "Amitabha Chattopadhyay and Harikumar, {K. G.}",
year = "1996",
month = "8",
day = "5",
doi = "10.1016/0014-5793(96)00733-8",
language = "English (US)",
volume = "391",
pages = "199--202",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength

T2 - Implications in receptor solubilization

AU - Chattopadhyay, Amitabha

AU - Harikumar, K. G.

PY - 1996/8/5

Y1 - 1996/8/5

N2 - The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1- propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT(1A)) from bovine brain hippocampal crude (native) membrane by CHAPS at pre- micellar concentration under high salt conditions.

AB - The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1- propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT(1A)) from bovine brain hippocampal crude (native) membrane by CHAPS at pre- micellar concentration under high salt conditions.

KW - 3-[(3-Cholamidopropyl)- dimethylammonio]-1-propane-sulfonate

KW - 5-HT(1A) receptor

KW - CHAPS

KW - Critical micelle concentration

KW - Ionic strength

KW - Solubilization

KW - Zwitterionic detergent

UR - http://www.scopus.com/inward/record.url?scp=0030570797&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030570797&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(96)00733-8

DO - 10.1016/0014-5793(96)00733-8

M3 - Article

C2 - 8706916

AN - SCOPUS:0030570797

VL - 391

SP - 199

EP - 202

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -