Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: Implications in receptor solubilization

Amitabha Chattopadhyay, K. G. Harikumar

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1- propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT(1A)) from bovine brain hippocampal crude (native) membrane by CHAPS at pre- micellar concentration under high salt conditions.

Original languageEnglish (US)
Pages (from-to)199-202
Number of pages4
JournalFEBS Letters
Volume391
Issue number1-2
DOIs
StatePublished - Aug 5 1996

Keywords

  • 3-[(3-Cholamidopropyl)- dimethylammonio]-1-propane-sulfonate
  • 5-HT(1A) receptor
  • CHAPS
  • Critical micelle concentration
  • Ionic strength
  • Solubilization
  • Zwitterionic detergent

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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