TY - JOUR
T1 - Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification
AU - Schweppe, Rebecca E.
AU - Klopper, Joshua P.
AU - Korch, Christopher
AU - Pugazhenthi, Umarani
AU - Benezra, Miriam
AU - Knauf, Jeffrey A.
AU - Fagin, James A.
AU - Marlow, Laura A.
AU - Copland, John A.
AU - Smallridge, Robert C.
AU - Haugen, Bryan R.
N1 - Funding Information:
This work was supported by ACS MRSG-06-193-01-TBE (to J.P.K.); University of Colorado Cancer Center Sequencing Core P30 CA046934 (to C.K.); NIH-CA50706 (to J.A.F.); CA72597 (to J.A.F.); a Translational and Integrative Medicine Research Grant from Memorial Sloan-Kettering Cancer Center (to J.A.F.); the Margot Rosenberg Pulitzer Foundation (to J.A.F.); Florida Department of Health Grant 07BB-02-12297 (to J.A.C.), grant for rare cancers from Dr. Ellis and Dona Brunton (to J.A.C.); NIH P30CA15083 (Cancer Center Support Grant) (to R.C.S.), Mayo Clinic Research Committee (to R.C.S.); NCI CA100560 (to B.R.H.), Mary Rossick Kern and Jerome H Kern Endowment (to B.R.H.).
PY - 2008/11
Y1 - 2008/11
N2 - Context: Cell lines derived from human cancers provide critical tools to study disease mechanisms and develop novel therapies. Recent reports indicate that up to 36% of cell lines are crossc-contaminated. Objective: We evaluated 40 reported thyroid cancer-derived cell lines using short tandem repeat and single nucleotide polymorphism array analysis. Results: Only 23 of 40 cell lines tested have unique genetic profiles. The following groups of cell lines are likely derivatives of the same cell line: BHP5-16, BHP17-10, BHP14-9, and NPA87; BHP2-7, BHP10-3, BHP7-13, and TPC1; KAT5, KAT10, KAT4, KAT7, KAT50, KAK1, ARO81-1, and MRO87-1; and K1 and K2. The unique cell lines include BCPAP, KTC1, TT2609-C02, FTC133, ML1, WRO82-1, 8505C, SW1736, Cal-62, T235, T238, Uhth-104, ACT-1, HTh74, KAT18, TTA1, FRO81-2, HTh7, C643, BHT101, and KTC-2. The misidentified cell lines included the DRO90-1, which matched the melanoma-derived cell line, A-375. The ARO81-1 and its derivatives matched the HT-29 colon cancer cell line, and the NPA87 and its derivatives matched the M14/MDA-MB-435S melanoma cell line. TTF-1 and Pax-8 mRNA levels were determined in the unique cell lines. Conclusions: Many of these human cell lines have been widely used in the thyroid cancer field for the past 20 yr and are not only redundant, but not of thyroid origin. These results emphasize the importance of cell line integrity, and provide the short tandem repeat profiles for a panel of thyroid cancer cell lines that can be used as a reference for comparison of cell lines from other laboratories.
AB - Context: Cell lines derived from human cancers provide critical tools to study disease mechanisms and develop novel therapies. Recent reports indicate that up to 36% of cell lines are crossc-contaminated. Objective: We evaluated 40 reported thyroid cancer-derived cell lines using short tandem repeat and single nucleotide polymorphism array analysis. Results: Only 23 of 40 cell lines tested have unique genetic profiles. The following groups of cell lines are likely derivatives of the same cell line: BHP5-16, BHP17-10, BHP14-9, and NPA87; BHP2-7, BHP10-3, BHP7-13, and TPC1; KAT5, KAT10, KAT4, KAT7, KAT50, KAK1, ARO81-1, and MRO87-1; and K1 and K2. The unique cell lines include BCPAP, KTC1, TT2609-C02, FTC133, ML1, WRO82-1, 8505C, SW1736, Cal-62, T235, T238, Uhth-104, ACT-1, HTh74, KAT18, TTA1, FRO81-2, HTh7, C643, BHT101, and KTC-2. The misidentified cell lines included the DRO90-1, which matched the melanoma-derived cell line, A-375. The ARO81-1 and its derivatives matched the HT-29 colon cancer cell line, and the NPA87 and its derivatives matched the M14/MDA-MB-435S melanoma cell line. TTF-1 and Pax-8 mRNA levels were determined in the unique cell lines. Conclusions: Many of these human cell lines have been widely used in the thyroid cancer field for the past 20 yr and are not only redundant, but not of thyroid origin. These results emphasize the importance of cell line integrity, and provide the short tandem repeat profiles for a panel of thyroid cancer cell lines that can be used as a reference for comparison of cell lines from other laboratories.
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U2 - 10.1210/jc.2008-1102
DO - 10.1210/jc.2008-1102
M3 - Article
C2 - 18713817
AN - SCOPUS:57349103343
SN - 0021-972X
VL - 93
SP - 4331
EP - 4341
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 11
ER -