Denervation augments alpha-2 but not alpha-1 adrenergic responses in canine saphenous veins

N. A. Flavahan, Virginia M Miller, L. L. Aarhus, P. M. Vanhoutte

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Experiments were performed in order to determine the influence of sympathetic denervation on alpha-1 and alpha-2 adrenergic responses in canine saphenous veins. In female dogs anesthetized with sodium pentobarbital, the left lumbar sympathetic chain was excised from L1 to L7. After a 3- to 5-week period, the left (denervated) and right (innervated) saphenous veins were removed, cut into rings and suspended for isometric tension recording in organ chambers filled with modified Krebs-Ringer bicarbonate solution. Denervation reduction significantly the norepinephrine content of the venous rings and the contractile responses evoked by the indirect sympathomimetic amine, tyramine. The contractile responses evoked by exogenous norepinephrine were augmented by denervation under control conditions (16.7-fold shift in concentration-effect curve) and also after inhibition of neuronal and extraneuronal uptake and beta adrenoceptors (3.8-fold shift in curve). Denervation increased the contractile responses evoked by the alpha-2 adrenergic agonist, UK 14,304 (5-fold shift in concentration-effect curve), but not those produced by the alpha-1 adrenergic agonist, phenylephrine. The selective augmentation of alpha-2 adrenergic responses by denervation may reflect the preferential innervation of alpha-2 adrenoceptors in the canine saphenous vein.

Original languageEnglish (US)
Pages (from-to)589-593
Number of pages5
JournalJournal of Pharmacology and Experimental Therapeutics
Volume240
Issue number2
StatePublished - 1987

Fingerprint

Saphenous Vein
Denervation
Adrenergic Agents
Canidae
Adrenergic Receptors
Adrenergic alpha-1 Receptor Agonists
Norepinephrine
Adrenergic alpha-2 Receptor Agonists
Sympathomimetics
Tyramine
Sympathectomy
Phenylephrine
Pentobarbital
Bicarbonates
Dogs

ASJC Scopus subject areas

  • Pharmacology

Cite this

Denervation augments alpha-2 but not alpha-1 adrenergic responses in canine saphenous veins. / Flavahan, N. A.; Miller, Virginia M; Aarhus, L. L.; Vanhoutte, P. M.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 240, No. 2, 1987, p. 589-593.

Research output: Contribution to journalArticle

@article{e2db2801afed44dc9e664beccd000958,
title = "Denervation augments alpha-2 but not alpha-1 adrenergic responses in canine saphenous veins",
abstract = "Experiments were performed in order to determine the influence of sympathetic denervation on alpha-1 and alpha-2 adrenergic responses in canine saphenous veins. In female dogs anesthetized with sodium pentobarbital, the left lumbar sympathetic chain was excised from L1 to L7. After a 3- to 5-week period, the left (denervated) and right (innervated) saphenous veins were removed, cut into rings and suspended for isometric tension recording in organ chambers filled with modified Krebs-Ringer bicarbonate solution. Denervation reduction significantly the norepinephrine content of the venous rings and the contractile responses evoked by the indirect sympathomimetic amine, tyramine. The contractile responses evoked by exogenous norepinephrine were augmented by denervation under control conditions (16.7-fold shift in concentration-effect curve) and also after inhibition of neuronal and extraneuronal uptake and beta adrenoceptors (3.8-fold shift in curve). Denervation increased the contractile responses evoked by the alpha-2 adrenergic agonist, UK 14,304 (5-fold shift in concentration-effect curve), but not those produced by the alpha-1 adrenergic agonist, phenylephrine. The selective augmentation of alpha-2 adrenergic responses by denervation may reflect the preferential innervation of alpha-2 adrenoceptors in the canine saphenous vein.",
author = "Flavahan, {N. A.} and Miller, {Virginia M} and Aarhus, {L. L.} and Vanhoutte, {P. M.}",
year = "1987",
language = "English (US)",
volume = "240",
pages = "589--593",
journal = "Journal of Pharmacology and Experimental Therapeutics",
issn = "0022-3565",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "2",

}

TY - JOUR

T1 - Denervation augments alpha-2 but not alpha-1 adrenergic responses in canine saphenous veins

AU - Flavahan, N. A.

AU - Miller, Virginia M

AU - Aarhus, L. L.

AU - Vanhoutte, P. M.

PY - 1987

Y1 - 1987

N2 - Experiments were performed in order to determine the influence of sympathetic denervation on alpha-1 and alpha-2 adrenergic responses in canine saphenous veins. In female dogs anesthetized with sodium pentobarbital, the left lumbar sympathetic chain was excised from L1 to L7. After a 3- to 5-week period, the left (denervated) and right (innervated) saphenous veins were removed, cut into rings and suspended for isometric tension recording in organ chambers filled with modified Krebs-Ringer bicarbonate solution. Denervation reduction significantly the norepinephrine content of the venous rings and the contractile responses evoked by the indirect sympathomimetic amine, tyramine. The contractile responses evoked by exogenous norepinephrine were augmented by denervation under control conditions (16.7-fold shift in concentration-effect curve) and also after inhibition of neuronal and extraneuronal uptake and beta adrenoceptors (3.8-fold shift in curve). Denervation increased the contractile responses evoked by the alpha-2 adrenergic agonist, UK 14,304 (5-fold shift in concentration-effect curve), but not those produced by the alpha-1 adrenergic agonist, phenylephrine. The selective augmentation of alpha-2 adrenergic responses by denervation may reflect the preferential innervation of alpha-2 adrenoceptors in the canine saphenous vein.

AB - Experiments were performed in order to determine the influence of sympathetic denervation on alpha-1 and alpha-2 adrenergic responses in canine saphenous veins. In female dogs anesthetized with sodium pentobarbital, the left lumbar sympathetic chain was excised from L1 to L7. After a 3- to 5-week period, the left (denervated) and right (innervated) saphenous veins were removed, cut into rings and suspended for isometric tension recording in organ chambers filled with modified Krebs-Ringer bicarbonate solution. Denervation reduction significantly the norepinephrine content of the venous rings and the contractile responses evoked by the indirect sympathomimetic amine, tyramine. The contractile responses evoked by exogenous norepinephrine were augmented by denervation under control conditions (16.7-fold shift in concentration-effect curve) and also after inhibition of neuronal and extraneuronal uptake and beta adrenoceptors (3.8-fold shift in curve). Denervation increased the contractile responses evoked by the alpha-2 adrenergic agonist, UK 14,304 (5-fold shift in concentration-effect curve), but not those produced by the alpha-1 adrenergic agonist, phenylephrine. The selective augmentation of alpha-2 adrenergic responses by denervation may reflect the preferential innervation of alpha-2 adrenoceptors in the canine saphenous vein.

UR - http://www.scopus.com/inward/record.url?scp=0023146743&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023146743&partnerID=8YFLogxK

M3 - Article

VL - 240

SP - 589

EP - 593

JO - Journal of Pharmacology and Experimental Therapeutics

JF - Journal of Pharmacology and Experimental Therapeutics

SN - 0022-3565

IS - 2

ER -