Dendritic cell microvilli: A novel membrane structure associated with the multifocal synapse and T-cell clustering

Phyllis J. Fisher, Peggy A. Bulur, Stanimir Vuk-Pavlovic, Franklyn G. Prendergast, Allan B Dietz

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Polarizing effects of productive dendritic cell (DC) - T-cell interactions on DC cy-toskeleton have been known in some detail, but the effects on DC membrane have been studied to a lesser extent. We found that T-cell incubation led to DC elongation and segregation of characteristic DC veils to the broader pole of the cell. On the opposite DC pole, we observed a novel membrane feature in the form of bundled microvilli. Each villus was approximately 100 nm in diameter and 600 to 1200 nm long. Microvilli exhibited high density of antigen-presenting molecules and costimulatory molecules and provided the physical basis for the multifocal immune synapse we observed during human DC and T-cell interactions. T cells preferentially bound to this site in clusters often contained both CD4 + and CD8 + T cells.

Original languageEnglish (US)
Pages (from-to)5037-5045
Number of pages9
JournalBlood
Volume112
Issue number13
DOIs
StatePublished - Dec 15 2008

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Membrane structures
T-cells
Microvilli
Synapses
Dendritic Cells
Cluster Analysis
T-Lymphocytes
Membranes
Cell Communication
Poles
Molecules
Cell Separation
Cell membranes
Elongation
Cell Membrane
Antigens

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Dendritic cell microvilli : A novel membrane structure associated with the multifocal synapse and T-cell clustering. / Fisher, Phyllis J.; Bulur, Peggy A.; Vuk-Pavlovic, Stanimir; Prendergast, Franklyn G.; Dietz, Allan B.

In: Blood, Vol. 112, No. 13, 15.12.2008, p. 5037-5045.

Research output: Contribution to journalArticle

Fisher, Phyllis J. ; Bulur, Peggy A. ; Vuk-Pavlovic, Stanimir ; Prendergast, Franklyn G. ; Dietz, Allan B. / Dendritic cell microvilli : A novel membrane structure associated with the multifocal synapse and T-cell clustering. In: Blood. 2008 ; Vol. 112, No. 13. pp. 5037-5045.
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