TY - JOUR
T1 - Dendritic cell microvilli
T2 - A novel membrane structure associated with the multifocal synapse and T-cell clustering
AU - Fisher, Phyllis J.
AU - Bulur, Peggy A.
AU - Vuk-Pavlovic, Stanimir
AU - Prendergast, Franklyn G.
AU - Dietz, Allan B.
PY - 2008/12/15
Y1 - 2008/12/15
N2 - Polarizing effects of productive dendritic cell (DC) - T-cell interactions on DC cy-toskeleton have been known in some detail, but the effects on DC membrane have been studied to a lesser extent. We found that T-cell incubation led to DC elongation and segregation of characteristic DC veils to the broader pole of the cell. On the opposite DC pole, we observed a novel membrane feature in the form of bundled microvilli. Each villus was approximately 100 nm in diameter and 600 to 1200 nm long. Microvilli exhibited high density of antigen-presenting molecules and costimulatory molecules and provided the physical basis for the multifocal immune synapse we observed during human DC and T-cell interactions. T cells preferentially bound to this site in clusters often contained both CD4+ and CD8+ T cells.
AB - Polarizing effects of productive dendritic cell (DC) - T-cell interactions on DC cy-toskeleton have been known in some detail, but the effects on DC membrane have been studied to a lesser extent. We found that T-cell incubation led to DC elongation and segregation of characteristic DC veils to the broader pole of the cell. On the opposite DC pole, we observed a novel membrane feature in the form of bundled microvilli. Each villus was approximately 100 nm in diameter and 600 to 1200 nm long. Microvilli exhibited high density of antigen-presenting molecules and costimulatory molecules and provided the physical basis for the multifocal immune synapse we observed during human DC and T-cell interactions. T cells preferentially bound to this site in clusters often contained both CD4+ and CD8+ T cells.
UR - http://www.scopus.com/inward/record.url?scp=58149402404&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149402404&partnerID=8YFLogxK
U2 - 10.1182/blood-2008-04-149526
DO - 10.1182/blood-2008-04-149526
M3 - Article
C2 - 18805966
AN - SCOPUS:58149402404
SN - 0006-4971
VL - 112
SP - 5037
EP - 5045
JO - Blood
JF - Blood
IS - 13
ER -