Dendritic cell microvilli: A novel membrane structure associated with the multifocal synapse and T-cell clustering

Phyllis J. Fisher, Peggy A. Bulur, Stanimir Vuk-Pavlovic, Franklyn G. Prendergast, Allan B. Dietz

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Polarizing effects of productive dendritic cell (DC) - T-cell interactions on DC cy-toskeleton have been known in some detail, but the effects on DC membrane have been studied to a lesser extent. We found that T-cell incubation led to DC elongation and segregation of characteristic DC veils to the broader pole of the cell. On the opposite DC pole, we observed a novel membrane feature in the form of bundled microvilli. Each villus was approximately 100 nm in diameter and 600 to 1200 nm long. Microvilli exhibited high density of antigen-presenting molecules and costimulatory molecules and provided the physical basis for the multifocal immune synapse we observed during human DC and T-cell interactions. T cells preferentially bound to this site in clusters often contained both CD4 + and CD8 + T cells.

Original languageEnglish (US)
Pages (from-to)5037-5045
Number of pages9
JournalBlood
Volume112
Issue number13
DOIs
StatePublished - Dec 15 2008

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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