Dendritic cell differentiation and proliferation

Enhancement by tumor necrosis factor-α

Robert S. Morrison, Julius M. Cruse, Huan D Wang, Robert E. Lewis

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Dendritic cells (DCs) are a diverse group of hematopoietic-derived cells that play a prominent role in initiating the body's immune response. Tumor necrosis factor-α (TNFα) aids CD34+ hematopoietic stem cells in the development of DCs. In this study, we aimed to further define the relationship between TNFα and DC maturation. CD34+ stem cells were isolated from umbilical cord blood and cultured using granulocyte- macrophage colony stimulating factor, stem cell factor, and varying concentrations of TNFα. An anti-TNF receptor 1 (anti-TNFR1) antibody was used to show the specificity of TNFα. Flow cytometry and light microscopy analyses were performed at days 0, 7, and 14 of culture, revealing mature DCs at all concentrations of TNFα by day 14, excluding those with anti-TNFR1 bound to the cell's TNF receptor 1. DCs possessed a characteristic veiled appearance and were consistent with a DC panel of surface markers. TNFα was essential to the development of DCs, as those with bound anti-TNFR1 were virtually unable to develop into DCs. Increasing TNFα enhanced the survival of culturing stem cells and resulted in a parallel increase in day 14 DCs. Although increases in TNFα produced more DCs, these cells were not as phenotypically mature, expressing less CD80 than those receiving only a single initial dosage of TNFα. These studies support the prevalence of large numbers of DCs under inflammatory conditions, such as the rheumatoid joint, where local concentrations of TNFα are high.

Original languageEnglish (US)
Pages (from-to)228-237
Number of pages10
JournalExperimental and Molecular Pathology
Volume75
Issue number3
DOIs
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Dendritic Cells
Cell Differentiation
Tumor Necrosis Factor-alpha
Cell Proliferation
Tumor Necrosis Factor Receptors
Stem cells
Stem Cells
Stem Cell Factor
Flow cytometry
Granulocyte-Macrophage Colony-Stimulating Factor
Hematopoietic Stem Cells
Fetal Blood
Optical microscopy
Microscopy
Flow Cytometry
Blood
Cross-Sectional Studies
Joints
Light
Antibodies

Keywords

  • CD antigens
  • CD80
  • CD86
  • Costimulatory molecules
  • Cytokines
  • Dendritic cells
  • Hematopoietic stem cells
  • T cell activation
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Dendritic cell differentiation and proliferation : Enhancement by tumor necrosis factor-α. / Morrison, Robert S.; Cruse, Julius M.; Wang, Huan D; Lewis, Robert E.

In: Experimental and Molecular Pathology, Vol. 75, No. 3, 12.2003, p. 228-237.

Research output: Contribution to journalArticle

Morrison, Robert S. ; Cruse, Julius M. ; Wang, Huan D ; Lewis, Robert E. / Dendritic cell differentiation and proliferation : Enhancement by tumor necrosis factor-α. In: Experimental and Molecular Pathology. 2003 ; Vol. 75, No. 3. pp. 228-237.
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