Delineation of the minimal encephalitogenic epitope of proteolipid protein peptide91-110 and critical residues required for induction of EAE in HLA-DR3 transgenic mice

Ashutosh K. Mangalam; Meenakshi Khare; Christopher J. Krco; Moses Rodriguez; Chella S. David

doi:10.1016/j.jneuroim.2004.12.012

Delineation of the minimal encephalitogenic epitope of proteolipid protein peptide_91-110 and critical residues required for induction of EAE in HLA-DR3 transgenic mice

Ashutosh K. Mangalam, Meenakshi Khare, Christopher J. Krco, Moses Rodriguez, Chella S. David

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Previously, we have reported that proteolipid protein (PLP) peptide 91-110 can induce experimental autoimmune encephalomyelitis (EAE) in HLA-DR3 transgenic (tg) mice. Here we, report that residues spanning 97-108 are the minimal epitope required for induction of EAE in DR3 mice. Utilizing a series of alanine-substituted peptides, positions 99, 101, 102, 103, 104, and 106 are identified as residues necessary for an immune response. Further analysis indicated that amino acid isoleucine (99), aspartate (102) and lysine (104) are anchor residues facilitating binding to HLA-DR3 molecules. These results may have applications in the future design of peptide based immunotherapy.

Original language	English (US)
Pages (from-to)	40-48
Number of pages	9
Journal	Journal of neuroimmunology
Volume	161
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2004.12.012
State	Published - Apr 2005

Keywords

EAE/MS
Epitopes
MHC
Proteolipid protein (PLP)
Transgenic mice

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

Access to Document

10.1016/j.jneuroim.2004.12.012

Cite this

Delineation of the minimal encephalitogenic epitope of proteolipid protein peptide_91-110 and critical residues required for induction of EAE in HLA-DR3 transgenic mice. / Mangalam, Ashutosh K.; Khare, Meenakshi; Krco, Christopher J. et al.
In: Journal of neuroimmunology, Vol. 161, No. 1-2, 04.2005, p. 40-48.

Research output: Contribution to journal › Article › peer-review

@article{a4a449bfbf1e48a99a9a7c37caae9f9c,

title = "Delineation of the minimal encephalitogenic epitope of proteolipid protein peptide91-110 and critical residues required for induction of EAE in HLA-DR3 transgenic mice",

abstract = "Previously, we have reported that proteolipid protein (PLP) peptide 91-110 can induce experimental autoimmune encephalomyelitis (EAE) in HLA-DR3 transgenic (tg) mice. Here we, report that residues spanning 97-108 are the minimal epitope required for induction of EAE in DR3 mice. Utilizing a series of alanine-substituted peptides, positions 99, 101, 102, 103, 104, and 106 are identified as residues necessary for an immune response. Further analysis indicated that amino acid isoleucine (99), aspartate (102) and lysine (104) are anchor residues facilitating binding to HLA-DR3 molecules. These results may have applications in the future design of peptide based immunotherapy.",

keywords = "EAE/MS, Epitopes, MHC, Proteolipid protein (PLP), Transgenic mice",

author = "Mangalam, {Ashutosh K.} and Meenakshi Khare and Krco, {Christopher J.} and Moses Rodriguez and David, {Chella S.}",

note = "Funding Information: The HLA transgenic mice were produced with support from NIH grant AI-14764. This work was funded by the National Institutes of Health grants P01-NS38468.",

year = "2005",

month = apr,

doi = "10.1016/j.jneuroim.2004.12.012",

language = "English (US)",

volume = "161",

pages = "40--48",

journal = "Journal of neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Delineation of the minimal encephalitogenic epitope of proteolipid protein peptide91-110 and critical residues required for induction of EAE in HLA-DR3 transgenic mice

AU - Mangalam, Ashutosh K.

AU - Khare, Meenakshi

AU - Krco, Christopher J.

AU - Rodriguez, Moses

AU - David, Chella S.

N1 - Funding Information: The HLA transgenic mice were produced with support from NIH grant AI-14764. This work was funded by the National Institutes of Health grants P01-NS38468.

PY - 2005/4

Y1 - 2005/4

N2 - Previously, we have reported that proteolipid protein (PLP) peptide 91-110 can induce experimental autoimmune encephalomyelitis (EAE) in HLA-DR3 transgenic (tg) mice. Here we, report that residues spanning 97-108 are the minimal epitope required for induction of EAE in DR3 mice. Utilizing a series of alanine-substituted peptides, positions 99, 101, 102, 103, 104, and 106 are identified as residues necessary for an immune response. Further analysis indicated that amino acid isoleucine (99), aspartate (102) and lysine (104) are anchor residues facilitating binding to HLA-DR3 molecules. These results may have applications in the future design of peptide based immunotherapy.

AB - Previously, we have reported that proteolipid protein (PLP) peptide 91-110 can induce experimental autoimmune encephalomyelitis (EAE) in HLA-DR3 transgenic (tg) mice. Here we, report that residues spanning 97-108 are the minimal epitope required for induction of EAE in DR3 mice. Utilizing a series of alanine-substituted peptides, positions 99, 101, 102, 103, 104, and 106 are identified as residues necessary for an immune response. Further analysis indicated that amino acid isoleucine (99), aspartate (102) and lysine (104) are anchor residues facilitating binding to HLA-DR3 molecules. These results may have applications in the future design of peptide based immunotherapy.

KW - EAE/MS

KW - Epitopes

KW - MHC

KW - Proteolipid protein (PLP)

KW - Transgenic mice

UR - http://www.scopus.com/inward/record.url?scp=14844290264&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14844290264&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2004.12.012

DO - 10.1016/j.jneuroim.2004.12.012

M3 - Article

C2 - 15748942

AN - SCOPUS:14844290264

SN - 0165-5728

VL - 161

SP - 40

EP - 48

JO - Journal of neuroimmunology

JF - Journal of neuroimmunology

IS - 1-2

ER -