Delineation of the minimal encephalitogenic epitope of proteolipid protein peptide91-110 and critical residues required for induction of EAE in HLA-DR3 transgenic mice

Ashutosh K. Mangalam, Meenakshi Khare, Christopher J. Krco, Moses Rodriguez, Chella S. David

Research output: Contribution to journalArticle

4 Scopus citations


Previously, we have reported that proteolipid protein (PLP) peptide 91-110 can induce experimental autoimmune encephalomyelitis (EAE) in HLA-DR3 transgenic (tg) mice. Here we, report that residues spanning 97-108 are the minimal epitope required for induction of EAE in DR3 mice. Utilizing a series of alanine-substituted peptides, positions 99, 101, 102, 103, 104, and 106 are identified as residues necessary for an immune response. Further analysis indicated that amino acid isoleucine (99), aspartate (102) and lysine (104) are anchor residues facilitating binding to HLA-DR3 molecules. These results may have applications in the future design of peptide based immunotherapy.

Original languageEnglish (US)
Pages (from-to)40-48
Number of pages9
JournalJournal of Neuroimmunology
Issue number1-2
StatePublished - Apr 2005



  • EAE/MS
  • Epitopes
  • MHC
  • Proteolipid protein (PLP)
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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