Delineation of the functional capacity of human neonatal lymphocytes

Judy B. Splawski, Diane F Jelinek, Peter E. Lipsky

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Neonatal T cell-B cell collaboration was investigated utilizing a system of T cell-dependent polyclonal B cell activation and Ig secretion. In this system, T cells activated by immobilized anti-CD3 provide a potent stimulus for Ig production by adult lymphocytes. By contrast, anti-CD3 stimulation of cord blood lymphocytes generated minimal numbers of Ig-secreting cells. Ig production by neonatal lymphocytes was enhanced by the addition of Staphylococcus aureus or secreted factors from mitogen-stimulated adult T cells. Supplementation with IL-2 resulted in the production of large amounts of IgM and small amounts of IgG and IgA, with less Ig produced than by comparable cultures of adult lymphocytes. Neonatal T cells proliferated and produced IL-2 in response to immobilized anti-CD3, and supported B cell proliferation and Ig secretion by adult B cells, although not as effectively as adult T cells. Supernatants from activated neonatal T cells were markedly limited in their capacity to support Ig production by adult B cells. Neonatal B cells could be induced to differentiate in response to anti-CD3-stimulated adult T cells. However, the amounts of IgG and IgA secreted were small compared to adult levels. These studies indicate a relative, but not absolute, functional deficiency of both neonatal B and T cells.

Original languageEnglish (US)
Pages (from-to)545-553
Number of pages9
JournalJournal of Clinical Investigation
Volume87
Issue number2
StatePublished - 1991
Externally publishedYes

Fingerprint

Lymphocytes
T-Lymphocytes
B-Lymphocytes
Immunoglobulin A
Interleukin-2
Immunoglobulin G
Fetal Blood
Mitogens
Immunoglobulin M
Staphylococcus aureus
Cell Proliferation

Keywords

  • CD3
  • Immunoglobulin
  • Interleukin 2
  • Neonatal B cells
  • T cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Delineation of the functional capacity of human neonatal lymphocytes. / Splawski, Judy B.; Jelinek, Diane F; Lipsky, Peter E.

In: Journal of Clinical Investigation, Vol. 87, No. 2, 1991, p. 545-553.

Research output: Contribution to journalArticle

Splawski, Judy B. ; Jelinek, Diane F ; Lipsky, Peter E. / Delineation of the functional capacity of human neonatal lymphocytes. In: Journal of Clinical Investigation. 1991 ; Vol. 87, No. 2. pp. 545-553.
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