Delineation of the functional capacity of human neonatal lymphocytes

J. B. Splawski, D. F. Jelinek, P. E. Lipsky

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Abstract

Neonatal T cell-B cell collaboration was investigated utilizing a system of T cell-dependent polyclonal B cell activation and Ig secretion. In this system, T cells activated by immobilized anti-CD3 provide a potent stimulus for Ig production by adult lymphocytes. By contrast, anti-CD3 stimulation of cord blood lymphocytes generated minimal numbers of Ig-secreting cells. Ig production by neonatal lymphocytes was enhanced by the addition of Staphylococcus aureus or secreted factors from mitogen-stimulated adult T cells. Supplementation with IL-2 resulted in the production of large amounts of IgM and small amounts of IgG and IgA, with less Ig produced than by comparable cultures of adult lymphocytes. Neonatal T cells proliferated and produced IL-2 in response to immobilized anti-CD3, and supported B cell proliferation and Ig secretion by adult B cells, although not as effectively as adult T cells. Supernatants from activated neonatal T cells were markedly limited in their capacity to support Ig production by adult B cells. Neonatal B cells could be induced to differentiate in response to anti-CD3-stimulated adult T cells. However, the amounts of IgG and IgA secreted were small compared to adult levels. These studies indicate a relative, but not absolute, functional deficiency of both neonatal B and T cells.

Original languageEnglish (US)
Pages (from-to)545-553
Number of pages9
JournalJournal of Clinical Investigation
Volume87
Issue number2
DOIs
StatePublished - Jan 1 1991

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Keywords

  • CD3
  • T cells
  • immunoglobulin
  • interleukin 2
  • neonatal B cells

ASJC Scopus subject areas

  • Medicine(all)

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