Deletion of p16 and p15 genes in brain tumors

Jin Jen, J. W. Harper, S. H. Bigner, D. D. Bigner, N. Papadopoulos, S. Markowitz, J. K V Willson, K. W. Kinzler, B. Vogelstein

Research output: Contribution to journalArticle

457 Citations (Scopus)

Abstract

We have used molecular genetic methods to examine the status of cell cycle-inhibitory genes in human brain tumors. We found that p16 and a neighboring gene, p15, were often homozygously deleted in glioblastoma multiformes but not in medulloblastomas or ependymomas. The deletions occurred in both primary tumors and their derived xenografts, but no intragenic mutations in either of the two genes were found. The p15 gene was expressed in a more widespread pattern in normal tissues than p16, but the products of both genes had similar capacities to bind to cyclin D-dependent kinases 4 and 6. These data suggest that the target of deletion in glioblastoma multiforme includes both p15 and p16 genes. The reason that homozygous deletions, rather than intragenic mutations, are so common in these tumors may be that deletion is a more efficient mechanism for simultaneous inactivation of both genes.

Original languageEnglish (US)
Pages (from-to)6353-6358
Number of pages6
JournalCancer Research
Volume54
Issue number24
StatePublished - 1994
Externally publishedYes

Fingerprint

p16 Genes
Brain Neoplasms
Glioblastoma
Genes
Cyclin-Dependent Kinase 6
Cyclin-Dependent Kinase 4
Cyclin D
cdc Genes
Ependymoma
Mutation
Medulloblastoma
Gene Silencing
Heterografts
Molecular Biology
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Jen, J., Harper, J. W., Bigner, S. H., Bigner, D. D., Papadopoulos, N., Markowitz, S., ... Vogelstein, B. (1994). Deletion of p16 and p15 genes in brain tumors. Cancer Research, 54(24), 6353-6358.

Deletion of p16 and p15 genes in brain tumors. / Jen, Jin; Harper, J. W.; Bigner, S. H.; Bigner, D. D.; Papadopoulos, N.; Markowitz, S.; Willson, J. K V; Kinzler, K. W.; Vogelstein, B.

In: Cancer Research, Vol. 54, No. 24, 1994, p. 6353-6358.

Research output: Contribution to journalArticle

Jen, J, Harper, JW, Bigner, SH, Bigner, DD, Papadopoulos, N, Markowitz, S, Willson, JKV, Kinzler, KW & Vogelstein, B 1994, 'Deletion of p16 and p15 genes in brain tumors', Cancer Research, vol. 54, no. 24, pp. 6353-6358.
Jen J, Harper JW, Bigner SH, Bigner DD, Papadopoulos N, Markowitz S et al. Deletion of p16 and p15 genes in brain tumors. Cancer Research. 1994;54(24):6353-6358.
Jen, Jin ; Harper, J. W. ; Bigner, S. H. ; Bigner, D. D. ; Papadopoulos, N. ; Markowitz, S. ; Willson, J. K V ; Kinzler, K. W. ; Vogelstein, B. / Deletion of p16 and p15 genes in brain tumors. In: Cancer Research. 1994 ; Vol. 54, No. 24. pp. 6353-6358.
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