Dehydroepiandrosterone sulfotransferase and estrogen sulfotransferase in human jejunum: Immunochemical characterization of individual variation

C. Her, I. A. Aksoy, C. L. Szumlanski, Richard M Weinshilboum

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sulfate conjugation, catalyzed by members of a gene superfamily of sulfotransferase (ST) enzymes, is an important metabolic pathway for many steroidt. Human tissues express at least five cytoplasmic ST enzymes, dehydroepiandrosterone (DHEA) ST, estrogen ST (EST) and three isoforms of phenol ST (PST). Individual variation in the expression of several of these enzymes is regulated by inheritance. We cloned cDNAs and genes and developed specific polyclonal antibodies for DHEA ST and EST in humans as a step toward molecular genetic experiments. DHEA ST and EST are both expressed in human small intestine. Therefore, we performed quantitative Western blot studies of 62 human jejunal mucosal samples obtained during clinically-Indicated surgery. Large individual variations in the quantity of DHEA ST and EST immunoreactive protein were detected that did not correlate significantly with time of tissue storage, patient age or gender. The jejunum was pathologically normal in 42 of these samples, while in 20 it was diseased. There were no significant differences between the two groups in immunoreactive DHEA ST or EST. Furthermore, immunoreactive DHEA ST and EST protein levels were not significantly correlated, indicating that they are regulated independently in human jejunum. These results represent a first step in the study of the molecular regulation of DHEA ST and EST expression in the human small intestine.

Original languageEnglish (US)
JournalJournal of Investigative Medicine
Volume44
Issue number3
StatePublished - 1996

Fingerprint

Jejunum
Estrogens
Sulfotransferases
Small Intestine
Enzymes
Arylsulfotransferase
Genes
Tissue
Metabolic Networks and Pathways
dehydroepiandrosterone sulfotransferase
estrone sulfotransferase
Surgery
Sulfates
Molecular Biology
Protein Isoforms
Proteins
Complementary DNA
Western Blotting
Antibodies
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{0429b1a9569e445ea488f913ec99cfb7,
title = "Dehydroepiandrosterone sulfotransferase and estrogen sulfotransferase in human jejunum: Immunochemical characterization of individual variation",
abstract = "Sulfate conjugation, catalyzed by members of a gene superfamily of sulfotransferase (ST) enzymes, is an important metabolic pathway for many steroidt. Human tissues express at least five cytoplasmic ST enzymes, dehydroepiandrosterone (DHEA) ST, estrogen ST (EST) and three isoforms of phenol ST (PST). Individual variation in the expression of several of these enzymes is regulated by inheritance. We cloned cDNAs and genes and developed specific polyclonal antibodies for DHEA ST and EST in humans as a step toward molecular genetic experiments. DHEA ST and EST are both expressed in human small intestine. Therefore, we performed quantitative Western blot studies of 62 human jejunal mucosal samples obtained during clinically-Indicated surgery. Large individual variations in the quantity of DHEA ST and EST immunoreactive protein were detected that did not correlate significantly with time of tissue storage, patient age or gender. The jejunum was pathologically normal in 42 of these samples, while in 20 it was diseased. There were no significant differences between the two groups in immunoreactive DHEA ST or EST. Furthermore, immunoreactive DHEA ST and EST protein levels were not significantly correlated, indicating that they are regulated independently in human jejunum. These results represent a first step in the study of the molecular regulation of DHEA ST and EST expression in the human small intestine.",
author = "C. Her and Aksoy, {I. A.} and Szumlanski, {C. L.} and Weinshilboum, {Richard M}",
year = "1996",
language = "English (US)",
volume = "44",
journal = "Journal of Investigative Medicine",
issn = "1081-5589",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Dehydroepiandrosterone sulfotransferase and estrogen sulfotransferase in human jejunum

T2 - Immunochemical characterization of individual variation

AU - Her, C.

AU - Aksoy, I. A.

AU - Szumlanski, C. L.

AU - Weinshilboum, Richard M

PY - 1996

Y1 - 1996

N2 - Sulfate conjugation, catalyzed by members of a gene superfamily of sulfotransferase (ST) enzymes, is an important metabolic pathway for many steroidt. Human tissues express at least five cytoplasmic ST enzymes, dehydroepiandrosterone (DHEA) ST, estrogen ST (EST) and three isoforms of phenol ST (PST). Individual variation in the expression of several of these enzymes is regulated by inheritance. We cloned cDNAs and genes and developed specific polyclonal antibodies for DHEA ST and EST in humans as a step toward molecular genetic experiments. DHEA ST and EST are both expressed in human small intestine. Therefore, we performed quantitative Western blot studies of 62 human jejunal mucosal samples obtained during clinically-Indicated surgery. Large individual variations in the quantity of DHEA ST and EST immunoreactive protein were detected that did not correlate significantly with time of tissue storage, patient age or gender. The jejunum was pathologically normal in 42 of these samples, while in 20 it was diseased. There were no significant differences between the two groups in immunoreactive DHEA ST or EST. Furthermore, immunoreactive DHEA ST and EST protein levels were not significantly correlated, indicating that they are regulated independently in human jejunum. These results represent a first step in the study of the molecular regulation of DHEA ST and EST expression in the human small intestine.

AB - Sulfate conjugation, catalyzed by members of a gene superfamily of sulfotransferase (ST) enzymes, is an important metabolic pathway for many steroidt. Human tissues express at least five cytoplasmic ST enzymes, dehydroepiandrosterone (DHEA) ST, estrogen ST (EST) and three isoforms of phenol ST (PST). Individual variation in the expression of several of these enzymes is regulated by inheritance. We cloned cDNAs and genes and developed specific polyclonal antibodies for DHEA ST and EST in humans as a step toward molecular genetic experiments. DHEA ST and EST are both expressed in human small intestine. Therefore, we performed quantitative Western blot studies of 62 human jejunal mucosal samples obtained during clinically-Indicated surgery. Large individual variations in the quantity of DHEA ST and EST immunoreactive protein were detected that did not correlate significantly with time of tissue storage, patient age or gender. The jejunum was pathologically normal in 42 of these samples, while in 20 it was diseased. There were no significant differences between the two groups in immunoreactive DHEA ST or EST. Furthermore, immunoreactive DHEA ST and EST protein levels were not significantly correlated, indicating that they are regulated independently in human jejunum. These results represent a first step in the study of the molecular regulation of DHEA ST and EST expression in the human small intestine.

UR - http://www.scopus.com/inward/record.url?scp=23544460120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23544460120&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:23544460120

VL - 44

JO - Journal of Investigative Medicine

JF - Journal of Investigative Medicine

SN - 1081-5589

IS - 3

ER -