Deformation-induced injury of alveolar epithelial cells: Effect of frequency, duration, and amplitude

Daniel J Tschumperlin, Jane Oswari, Susan S. Margulies

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the possible role of alveolar epithelial deformation in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. We report here a study of the relative effect of deformation frequency, duration, and amplitude on cell viability. We exposed rat primary alveolar epithelial cells to a variety of biaxial stretch protocols, and assessed deformation-induced cell injury quantitatively, using a fluorescent cell viability assay. Deformation-induced injury was found to depend on repetitive stretching, with cyclic deformations significantly more damaging than tonically held deformations. In cyclically deformed cells, injury occurred rapidly, with the majority of cell death occurring during the first 5 min of deformation. Deformation-induced injury was increased with the frequency of sustained cyclic deformations, but was not dependent on the deformation rate during a single stretch. Reducing the amplitude of cell deformations by superimposing small cyclic deformations on a tonic deformation significantly reduced cell death as compared with large-amplitude deformations with the same peak deformation.

Original languageEnglish (US)
Pages (from-to)357-362
Number of pages6
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume162
Issue number2 I
StatePublished - 2000
Externally publishedYes

Fingerprint

Alveolar Epithelial Cells
Ventilator-Induced Lung Injury
Cell Survival
Wounds and Injuries
Cell Death

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Deformation-induced injury of alveolar epithelial cells : Effect of frequency, duration, and amplitude. / Tschumperlin, Daniel J; Oswari, Jane; Margulies, Susan S.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 162, No. 2 I, 2000, p. 357-362.

Research output: Contribution to journalArticle

@article{6c0a5bbd1a2f4b74b0c0322dd03ef1af,
title = "Deformation-induced injury of alveolar epithelial cells: Effect of frequency, duration, and amplitude",
abstract = "The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the possible role of alveolar epithelial deformation in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. We report here a study of the relative effect of deformation frequency, duration, and amplitude on cell viability. We exposed rat primary alveolar epithelial cells to a variety of biaxial stretch protocols, and assessed deformation-induced cell injury quantitatively, using a fluorescent cell viability assay. Deformation-induced injury was found to depend on repetitive stretching, with cyclic deformations significantly more damaging than tonically held deformations. In cyclically deformed cells, injury occurred rapidly, with the majority of cell death occurring during the first 5 min of deformation. Deformation-induced injury was increased with the frequency of sustained cyclic deformations, but was not dependent on the deformation rate during a single stretch. Reducing the amplitude of cell deformations by superimposing small cyclic deformations on a tonic deformation significantly reduced cell death as compared with large-amplitude deformations with the same peak deformation.",
author = "Tschumperlin, {Daniel J} and Jane Oswari and Margulies, {Susan S.}",
year = "2000",
language = "English (US)",
volume = "162",
pages = "357--362",
journal = "American Journal of Respiratory and Critical Care Medicine",
issn = "1073-449X",
publisher = "American Thoracic Society",
number = "2 I",

}

TY - JOUR

T1 - Deformation-induced injury of alveolar epithelial cells

T2 - Effect of frequency, duration, and amplitude

AU - Tschumperlin, Daniel J

AU - Oswari, Jane

AU - Margulies, Susan S.

PY - 2000

Y1 - 2000

N2 - The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the possible role of alveolar epithelial deformation in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. We report here a study of the relative effect of deformation frequency, duration, and amplitude on cell viability. We exposed rat primary alveolar epithelial cells to a variety of biaxial stretch protocols, and assessed deformation-induced cell injury quantitatively, using a fluorescent cell viability assay. Deformation-induced injury was found to depend on repetitive stretching, with cyclic deformations significantly more damaging than tonically held deformations. In cyclically deformed cells, injury occurred rapidly, with the majority of cell death occurring during the first 5 min of deformation. Deformation-induced injury was increased with the frequency of sustained cyclic deformations, but was not dependent on the deformation rate during a single stretch. Reducing the amplitude of cell deformations by superimposing small cyclic deformations on a tonic deformation significantly reduced cell death as compared with large-amplitude deformations with the same peak deformation.

AB - The onset of ventilator-induced lung injury (VILI) is linked to a number of possible mechanisms. To isolate the possible role of alveolar epithelial deformation in the development of VILI, we have developed an in vitro system in which changes in alveolar epithelial cell viability can be measured after exposure to tightly controlled and physiologically relevant deformations. We report here a study of the relative effect of deformation frequency, duration, and amplitude on cell viability. We exposed rat primary alveolar epithelial cells to a variety of biaxial stretch protocols, and assessed deformation-induced cell injury quantitatively, using a fluorescent cell viability assay. Deformation-induced injury was found to depend on repetitive stretching, with cyclic deformations significantly more damaging than tonically held deformations. In cyclically deformed cells, injury occurred rapidly, with the majority of cell death occurring during the first 5 min of deformation. Deformation-induced injury was increased with the frequency of sustained cyclic deformations, but was not dependent on the deformation rate during a single stretch. Reducing the amplitude of cell deformations by superimposing small cyclic deformations on a tonic deformation significantly reduced cell death as compared with large-amplitude deformations with the same peak deformation.

UR - http://www.scopus.com/inward/record.url?scp=0033837424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033837424&partnerID=8YFLogxK

M3 - Article

C2 - 10934053

AN - SCOPUS:0033837424

VL - 162

SP - 357

EP - 362

JO - American Journal of Respiratory and Critical Care Medicine

JF - American Journal of Respiratory and Critical Care Medicine

SN - 1073-449X

IS - 2 I

ER -