Defining topological similarities among ion transport proteins with anti-amiloride antibodies

T. Kieber-Emmons, C. Lin, K. V. Prammer, A. Villalobos, Farhad Kosari, T. R. Kleyman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The structural features of amiloride binding sites on amiloride-sensitive transport proteins have received limited characterization. An antibody that recognizes limited regions of amiloride and can mimic, in binding specificity, certain amiloride-sensitive transport proteins was used as a model to elucidate potential amino acid residue relationships that might define putative amiloride contact sites. Analysis of the structure of this antibody has allowed us to identify sequence relationships among several Na + selective transport proteins. A structure-based relational database was employed to re-examine sequence homologies among these ion transport proteins. A search of the protein sequence databank identified representative amino acid tracts among amiloride sensitive proteins involving planar residues that might be involved in interacting with amiloride. Computer models of sites within transmembrane domains of NHE1 and NHE2 isoforms of the Na +/H + exchanger reflective of these planar tracts indicate that amiloride probably spans two helices for interaction with the Na +/H + exchanger. Structural analysis of this monoclonal anti-amiloride antibody appears to mimic some of the salient features of amiloride binding sites on these proteins.

Original languageEnglish (US)
Pages (from-to)956-964
Number of pages9
JournalKidney International
Volume48
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Amiloride
Ion Transport
Anti-Idiotypic Antibodies
Carrier Proteins
Sodium-Hydrogen Antiporter
Binding Sites
Databases
Amino Acids
Proteins
Antibodies
Sequence Homology
Computer Simulation
Protein Isoforms
Monoclonal Antibodies

ASJC Scopus subject areas

  • Nephrology

Cite this

Kieber-Emmons, T., Lin, C., Prammer, K. V., Villalobos, A., Kosari, F., & Kleyman, T. R. (1995). Defining topological similarities among ion transport proteins with anti-amiloride antibodies. Kidney International, 48(4), 956-964.

Defining topological similarities among ion transport proteins with anti-amiloride antibodies. / Kieber-Emmons, T.; Lin, C.; Prammer, K. V.; Villalobos, A.; Kosari, Farhad; Kleyman, T. R.

In: Kidney International, Vol. 48, No. 4, 1995, p. 956-964.

Research output: Contribution to journalArticle

Kieber-Emmons, T, Lin, C, Prammer, KV, Villalobos, A, Kosari, F & Kleyman, TR 1995, 'Defining topological similarities among ion transport proteins with anti-amiloride antibodies', Kidney International, vol. 48, no. 4, pp. 956-964.
Kieber-Emmons T, Lin C, Prammer KV, Villalobos A, Kosari F, Kleyman TR. Defining topological similarities among ion transport proteins with anti-amiloride antibodies. Kidney International. 1995;48(4):956-964.
Kieber-Emmons, T. ; Lin, C. ; Prammer, K. V. ; Villalobos, A. ; Kosari, Farhad ; Kleyman, T. R. / Defining topological similarities among ion transport proteins with anti-amiloride antibodies. In: Kidney International. 1995 ; Vol. 48, No. 4. pp. 956-964.
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