Deficiency of polycystin-2 reduces Ca2+ channel activity and cell proliferation in ADPKD lymphoblastoid cells.

Gianluca Aguiari, Manuela Banzi, Stefania Gessi, Yiqiang Cai, Emanuela Zeggio, Elisa Manzati, Roberta Piva, Elisabetta Lambertini, Luisa Ferrari, Dorien J. Peters, Francesco Lanza, Peter C. Harris, Pier Andrea Borea, Stefan Somlo, Laura Del Senno

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51 Scopus citations

Abstract

Polycystin-2 (PC2), encoded by the PKD2 gene, mutated in 10-15% of autosomal-dominant polycystic kidney disease (ADPKD) patients, is a Ca2+-permeable cation channel present in kidney epithelia and other tissues. As PC2 was found expressed in B-lymphoblastoid cells (LCLs) and Ca2+ signaling pathways are important regulators of B cell function activities, we investigated whether PC2 plays some role in B-LCLs. In LCLs, PC2 was found mainly in ER membranes but ~8 times less than in kidney HEK293 cells. The same reductions were found in PKD2 and PKD1 RNA; thus, PKD genes maintained, in LCLs, the same reciprocal proportion as they do in kidney cells. In LCLs obtained from subjects carrying PKD2 mutations (PKD2-LCLs) and showing reduced PC2 levels, intracellular Ca2+ concentrations evoked by platelet-activating factor (PAF), were significantly lower than in non-PKD-LCLs. This reduction was also found in PKD1-LCLs but without PC2 reductions. Likewise, cell proliferation, which is controlled by Ca2+, was reduced in PKD2- and PKD1-LCLs. Moreover, in LCLs with PKD2 nonsense mutations, aminoglycoside antibiotics reduced the PC2 defect by promoting readthrough of stop codons. Therefore, PC2 and PC1 are functionally expressed in LCLs, which provide a model, easily obtainable from ADPKD patients, to study PKD gene expression and function.

Original languageEnglish (US)
Pages (from-to)884-886
Number of pages3
JournalThe FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume18
Issue number7
StatePublished - May 2004

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Aguiari, G., Banzi, M., Gessi, S., Cai, Y., Zeggio, E., Manzati, E., Piva, R., Lambertini, E., Ferrari, L., Peters, D. J., Lanza, F., Harris, P. C., Borea, P. A., Somlo, S., & Del Senno, L. (2004). Deficiency of polycystin-2 reduces Ca2+ channel activity and cell proliferation in ADPKD lymphoblastoid cells. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 18(7), 884-886.