Decreased peripheral nerve damage after ischemia-reperfusion injury in mice lacking TNF-alpha

Yanping Wang, Nobutoshi Kawamura, James D. Schmelzer, Ann M. Schmeichel, Phillip A. Low

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We sought to explore the role of tumor necrosis factor-alpha (TNF-α) in the pathogenesis of peripheral nerve ischemia-reperfusion (IR) injury. We established an ischemia-reperfusion model in wild type (WT) and TNF-α knockout (KO) mice. Electrophysiology, behavioral score and morphological indices (edema and ischemic fiber degeneration [IFD]) were examined to determine the influence of TNF-α on peripheral nerve structure and function following ischemia followed by reperfusion. TNF-α and nuclear factor-kappa B (NF-κB) expression were evaluated using immunohistochemistry. TNF-α KO mice, compared to WT had, in sciatic nerve, marked improvement in nerve pathology. This is a region subject to moderate ischemia-reperfusion injury. There was also a significant improvement in electrophysiological and some behavioral indices. TNF-α and NF-κB expression were abundant in sciatic-tibial nerves of WT mice subjected to IR, but there was less, or complete lack of, expression in ischemic nerve of TNF-α KO mice. We conclude that TNF-α plays an essential role in the pathogenesis of peripheral nerve ischemia-reperfusion injury, possibly partly through the activation of NF-κB.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalJournal of the neurological sciences
Volume267
Issue number1-2
DOIs
StatePublished - Apr 15 2008

Keywords

  • Ischemia-reperfusion (IR) injury
  • Nuclear factor-kappa B (NF-κB)
  • Peripheral nerve
  • Tumor necrosis factor-alpha (TNF-α)

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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