Decreased local and systemic levels of sFRP3 protein in osteosarcoma patients

Dalibel Bravo; Ahmet Salduz; Kristen L. Shogren; Madison N. Okuno; James L. Herrick; Scott H. Okuno; Mario Galindo; Andre J. van Wijnen; Michael J. Yaszemski; Avudaiappan Maran

doi:10.1016/j.gene.2018.06.059

Decreased local and systemic levels of sFRP3 protein in osteosarcoma patients

Dalibel Bravo, Ahmet Salduz, Kristen L. Shogren, Madison N. Okuno, James L. Herrick, Scott H. Okuno, Mario Galindo, Andre J. van Wijnen, Michael J. Yaszemski, Avudaiappan Maran

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Osteosarcoma is a malignant bone tumor that occurs mainly in children and adolescents. Because Wnt signaling has been implicated in the pathogenesis of osteosarcoma, we have investigated the circulating and local levels of the Wnt antagonist protein, Secreted Frizzled Related Protein (sFRP) 3, in osteosarcoma patients. Enzyme linked immunosorbent assay (ELISA) analysis of 67 osteosarcoma and age-matched non-diseased control sera showed that sFPR3 protein levels were significantly lower in osteosarcoma than in normal. Analysis of tumor and adjacent normal tissues (9 pairs) from osteosarcoma patients showed a decrease in sFRP3 expression in 5 out of 9 tumor samples compared to normal tissues. Furthermore, immunohistochemical analysis of tissue microarray revealed a significant decrease in sFRP3 levels in tumor compared to normal bone. RNA sequencing analysis in osteosarcoma cells shows suppression of sFRP3 and concomitant expression of multiple Wnt family members mediating canonical or non-canonical Wnt signaling. Taken together, our findings show that the systemic and local levels of sFRP3 protein are downregulated in osteosarcoma and sFRP3 levels could be explored further in the diagnosis and the care of osteosarcoma patients.

Original language	English (US)
Pages (from-to)	1-7
Number of pages	7
Journal	Gene
Volume	674
DOIs	https://doi.org/10.1016/j.gene.2018.06.059
State	Published - Oct 20 2018

Keywords

Frzb
Osteosarcoma
Wnt
sFRP3

ASJC Scopus subject areas

Genetics

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10.1016/j.gene.2018.06.059

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title = "Decreased local and systemic levels of sFRP3 protein in osteosarcoma patients",

abstract = "Osteosarcoma is a malignant bone tumor that occurs mainly in children and adolescents. Because Wnt signaling has been implicated in the pathogenesis of osteosarcoma, we have investigated the circulating and local levels of the Wnt antagonist protein, Secreted Frizzled Related Protein (sFRP) 3, in osteosarcoma patients. Enzyme linked immunosorbent assay (ELISA) analysis of 67 osteosarcoma and age-matched non-diseased control sera showed that sFPR3 protein levels were significantly lower in osteosarcoma than in normal. Analysis of tumor and adjacent normal tissues (9 pairs) from osteosarcoma patients showed a decrease in sFRP3 expression in 5 out of 9 tumor samples compared to normal tissues. Furthermore, immunohistochemical analysis of tissue microarray revealed a significant decrease in sFRP3 levels in tumor compared to normal bone. RNA sequencing analysis in osteosarcoma cells shows suppression of sFRP3 and concomitant expression of multiple Wnt family members mediating canonical or non-canonical Wnt signaling. Taken together, our findings show that the systemic and local levels of sFRP3 protein are downregulated in osteosarcoma and sFRP3 levels could be explored further in the diagnosis and the care of osteosarcoma patients.",

keywords = "Frzb, Osteosarcoma, Wnt, sFRP3",

author = "Dalibel Bravo and Ahmet Salduz and Shogren, {Kristen L.} and Okuno, {Madison N.} and Herrick, {James L.} and Okuno, {Scott H.} and Mario Galindo and {van Wijnen}, {Andre J.} and Yaszemski, {Michael J.} and Avudaiappan Maran",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2018",

month = oct,

day = "20",

doi = "10.1016/j.gene.2018.06.059",

language = "English (US)",

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T1 - Decreased local and systemic levels of sFRP3 protein in osteosarcoma patients

AU - Bravo, Dalibel

AU - Salduz, Ahmet

AU - Shogren, Kristen L.

AU - Okuno, Madison N.

AU - Herrick, James L.

AU - Okuno, Scott H.

AU - Galindo, Mario

AU - van Wijnen, Andre J.

AU - Yaszemski, Michael J.

AU - Maran, Avudaiappan

PY - 2018/10/20

Y1 - 2018/10/20

N2 - Osteosarcoma is a malignant bone tumor that occurs mainly in children and adolescents. Because Wnt signaling has been implicated in the pathogenesis of osteosarcoma, we have investigated the circulating and local levels of the Wnt antagonist protein, Secreted Frizzled Related Protein (sFRP) 3, in osteosarcoma patients. Enzyme linked immunosorbent assay (ELISA) analysis of 67 osteosarcoma and age-matched non-diseased control sera showed that sFPR3 protein levels were significantly lower in osteosarcoma than in normal. Analysis of tumor and adjacent normal tissues (9 pairs) from osteosarcoma patients showed a decrease in sFRP3 expression in 5 out of 9 tumor samples compared to normal tissues. Furthermore, immunohistochemical analysis of tissue microarray revealed a significant decrease in sFRP3 levels in tumor compared to normal bone. RNA sequencing analysis in osteosarcoma cells shows suppression of sFRP3 and concomitant expression of multiple Wnt family members mediating canonical or non-canonical Wnt signaling. Taken together, our findings show that the systemic and local levels of sFRP3 protein are downregulated in osteosarcoma and sFRP3 levels could be explored further in the diagnosis and the care of osteosarcoma patients.

AB - Osteosarcoma is a malignant bone tumor that occurs mainly in children and adolescents. Because Wnt signaling has been implicated in the pathogenesis of osteosarcoma, we have investigated the circulating and local levels of the Wnt antagonist protein, Secreted Frizzled Related Protein (sFRP) 3, in osteosarcoma patients. Enzyme linked immunosorbent assay (ELISA) analysis of 67 osteosarcoma and age-matched non-diseased control sera showed that sFPR3 protein levels were significantly lower in osteosarcoma than in normal. Analysis of tumor and adjacent normal tissues (9 pairs) from osteosarcoma patients showed a decrease in sFRP3 expression in 5 out of 9 tumor samples compared to normal tissues. Furthermore, immunohistochemical analysis of tissue microarray revealed a significant decrease in sFRP3 levels in tumor compared to normal bone. RNA sequencing analysis in osteosarcoma cells shows suppression of sFRP3 and concomitant expression of multiple Wnt family members mediating canonical or non-canonical Wnt signaling. Taken together, our findings show that the systemic and local levels of sFRP3 protein are downregulated in osteosarcoma and sFRP3 levels could be explored further in the diagnosis and the care of osteosarcoma patients.

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Decreased local and systemic levels of sFRP3 protein in osteosarcoma patients

Abstract

Keywords

ASJC Scopus subject areas

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