TY - JOUR
T1 - Deconvolution analysis of hormone data
AU - Veldhuis, Johannes D.
AU - Johnson, Michael L.
N1 - Funding Information:
This research was supported by National Institutes of Health Grant GM-17452.
Funding Information:
We thank Patsy Craig for preparation of the manuscript and Paula P. Azimi for the artwork. This work was supported in part by National Institutes of Health Grant RR 00847 to the Clinical Research Center of the University of Virginia, Grant RCDA 1 KO4 HD00634 (J.D.V.), Grant GM-28928 (M.L.J.), the Diabetes and Endocrinology Research Center Grant NIH DK-38942, the NIH-supported Clinfo Data Reduction Systems, the Pratt Foundation, the University of Virginia Academic Enhancement Fund, and the National Science Foundation Science Center for Biological Timing (DIR-8920162).
PY - 1992/1/1
Y1 - 1992/1/1
N2 - This chapter evaluates methods of deconvolution analysis that have been applied to a specific topic in biology—namely, the temporal behavior of hormones, metabolites, and substrates in a sampled fluid compartment, such as blood. Such analyses have a significant place in this area of biology, because the time structure of the hormone, metabolite, or substrate concentration signal conveys important information to the target tissue, yields insights into systems regulation, and subserves the homeostasis of an organism. Deconvolution is a mathematical technique that has been utilized in the physical, applied, and natural sciences as a method for estimating the particular behavior of one or more component processes contributing to an observed outcome. Deconvolution would attempt to reconstruct the initial shock impulse from time records of the remote signal. In the field of spectroscopy, the intensity of an emitted wavelength of light measured at some remote point from the light source is influenced by the energy properties of the initial fluorescent signal and attenuation of the emitted signal as it travels to the point of observation. Deconvolution attempts to recover estimates of the intensity of the original fluorescent discharge. Spontaneous variations in measured concentrations of a metabolite, substrate, or hormone over time are controlled by at least two distinct processes (in addition to effects of “noise” in the system), which are as follows: (1) the rate of entry of the constituent into the blood compartment and (2) the magnitude and type of elimination kinetics serving to remove the compound from the circulation. The measurement of a hormone, metabolite, or substrate in a body fluid is always attended by some degree of assay imprecision, is restrained by some level of assay sensitivity (minimal detectable or limiting hormone concentration), and is subject to some particular specificity (the extent to which the assay correctly reports amounts of the substance of interest, and conversely the extent to which it does not falsely report the presence of other substances).
AB - This chapter evaluates methods of deconvolution analysis that have been applied to a specific topic in biology—namely, the temporal behavior of hormones, metabolites, and substrates in a sampled fluid compartment, such as blood. Such analyses have a significant place in this area of biology, because the time structure of the hormone, metabolite, or substrate concentration signal conveys important information to the target tissue, yields insights into systems regulation, and subserves the homeostasis of an organism. Deconvolution is a mathematical technique that has been utilized in the physical, applied, and natural sciences as a method for estimating the particular behavior of one or more component processes contributing to an observed outcome. Deconvolution would attempt to reconstruct the initial shock impulse from time records of the remote signal. In the field of spectroscopy, the intensity of an emitted wavelength of light measured at some remote point from the light source is influenced by the energy properties of the initial fluorescent signal and attenuation of the emitted signal as it travels to the point of observation. Deconvolution attempts to recover estimates of the intensity of the original fluorescent discharge. Spontaneous variations in measured concentrations of a metabolite, substrate, or hormone over time are controlled by at least two distinct processes (in addition to effects of “noise” in the system), which are as follows: (1) the rate of entry of the constituent into the blood compartment and (2) the magnitude and type of elimination kinetics serving to remove the compound from the circulation. The measurement of a hormone, metabolite, or substrate in a body fluid is always attended by some degree of assay imprecision, is restrained by some level of assay sensitivity (minimal detectable or limiting hormone concentration), and is subject to some particular specificity (the extent to which the assay correctly reports amounts of the substance of interest, and conversely the extent to which it does not falsely report the presence of other substances).
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U2 - 10.1016/0076-6879(92)10028-C
DO - 10.1016/0076-6879(92)10028-C
M3 - Article
C2 - 1584051
AN - SCOPUS:0026724547
SN - 0076-6879
VL - 210
SP - 539
EP - 575
JO - Methods in enzymology
JF - Methods in enzymology
IS - C
ER -